A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress
A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress
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DOI：
10.1016/j.cclet.2017.08.021
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发表时间：
2017-10-01
影响因子：
9.1
通讯作者：
Zhang, Xiao-Bing
中科院分区：
文献类型：
--
作者：
Cheng, Dan;Pan, Yue;Zhang, Xiao-Bing
本文开发了一种用于硒醇检测的新型超低背景荧光荧光探针（Rhod-Sec），其显示出380倍的关闭荧光响应，并且无毒的Rhod-Sec非常适合活细胞中外源和内源硒醇的检测和成像。它还可用于通过 LPS 诱导细胞抗氧化来可视化 HepG2 细胞中硒醇的波动。 (C) 2017 中国化学会、中国医学科学院药物研究所。由 Elsevier B.V. 出版。保留所有权利。
A new fluorescent probe (Rhod-Sec) for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.