Review; Agriculture and Environmental Biotechnology Succinic Acid Production from Xylose Mother Liquor by Recombinant Escherichia Coli Strain
Review; Agriculture and Environmental Biotechnology Succinic Acid Production from Xylose Mother Liquor by Recombinant Escherichia Coli Strain
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通讯作者：
Honghui Wang;Jiachuan Pan;Jing Wang;Nan Wang;Jie Zhang;Qiang Li;Danshi Wang;Xiaohua Zhou
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作者：
Honghui Wang;Jiachuan Pan;Jing Wang;Nan Wang;Jie Zhang;Qiang Li;Danshi Wang;Xiaohua Zhou
琥珀酸（1,4-丁二酸）被认为是重要的化学原料之一。木糖母液是木糖醇生物精炼行业中丰富的工业废渣。本研究以重组大肠杆菌SD121为原料，利用木糖母液生产琥珀酸，并采用响应面法对发酵培养基进行优化。琥珀酸发酵的最佳条件为：初始总糖82.62 g L ¡1、MgCO 3 42.27 g L ¡1、酵母抽提物17.84 g L ¡1。 84 h后琥珀酸的最大产量为52.09 § 0.21 g L ¡1，总糖产量为0.63 § 0.03 g g ¡1，接近预测值（53.18 g L ¡1）。是基础培养基产量的 1.78 倍。这是第一篇关于重组大肠杆菌菌株从木糖母液生产琥珀酸的报告，并使用响应面方法进行培养基优化。这项工作表明，木糖母液可以作为重组大肠杆菌菌株经济生产琥珀酸的替代底物。简介 琥珀酸，一种 C 4-二羧酸，也称为琥珀酸，是三羧酸循环 (TCA) 的中间体或无氧代谢的最终产物。[1] 琥珀酸被美国能源部列为 12 种平台生物基化学品中最有前途的一种。[2]它被用作食品、农业和制药行业的特种化学品，是生产各种高附加值衍生物的潜在平台化学品，包括1,4-丁二醇（BDO）、四氢呋喃（THF）、g-丁内酯（GBL）、琥珀酰亚胺，特别是可生物降解材料聚丁二酸丁二醇酯（PBS）。[3,4]传统上，琥珀酸是通过石油基工艺生产的，导致资源枯竭、环境污染、成本高等诸多问题。[5]生物质是最丰富、成本最低的可再生资源之一。在过去的几十年中，人们对利用天然生物质生产琥珀酸的兴趣有所增加，[6] 而生物质微生物发酵生产琥珀酸已被证明是一种环境友好且节能的工艺。[1,7] 用于生产琥珀酸的主要菌株包括 Actinobacillus suc-cinogenes、[8] Mannheimia succinici Producens、[9] Anae-robiospirillum succinici Producen，[10]和重组大肠杆菌，[11，12]谷氨酸棒杆菌，酿酒酵母和Basfia succinici Producens。
Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L ¡1 total initial sugars, 42.27 g L ¡1 MgCO 3 and 17.84 g L ¡1 yeast extract. The maximum production of succinic acid was 52.09 § 0.21 g L ¡1 after 84 h with a yield of 0.63 § 0.03 g g ¡1 total sugar, approaching the predicted value (53.18 g L ¡1). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains. Introduction Succinic acid, a C 4-dicarboxylic acid also known as amber acid, is an intermediate in the tricarboxylic acid cycle (TCA) or as an end product of anaerobic metabolism .[1] Succinic acid is classified as the most promising one among 12 platform bio-based chemicals by the US Department of Energy.[2] It is used as a special chemical in food, agricultural and pharmaceutical industries and is a potential platform chemical for the production of various high value-added derivatives, including 1,4-butane-diol (BDO), tetrahydrofuran (THF), g-butyrolactone (GBL), succinimide and especially the biodegradable material poly-butylenessuccinate (PBS).[3,4] Traditionally , succinic acid is produced via a petroleum-based process , which leads to many problems, such as resource depletion, environmental pollution and higher cost.[5] Biomass is one of the most abundant and low-cost renewable resource. The interest in producing succinic acid from natural biomass has increased during the last decades ,[6] while microbial fermentation of biomass to suc-cinic acid has been testified as an environmentally friendly and energy-efficient process.[1,7] Major strains used to produce succinic acid include Actinobacillus suc-cinogenes,[8] Mannheimia succiniciproducens,[9] Anae-robiospirillum succiniciproducen,[10] and recombinant Escherichia coli,[11,12] Corynebacterium glutamicum, Saccharomyces cerevisiae and Basfia succiniciproducens.