HIGH-PRESSURE INFRARED STUDY OF PHOSPHATIDYLSERINE BILAYERS AND THEIR INTERACTIONS WITH THE LOCAL-ANESTHETIC TETRACAINE
HIGH-PRESSURE INFRARED STUDY OF PHOSPHATIDYLSERINE BILAYERS AND THEIR INTERACTIONS WITH THE LOCAL-ANESTHETIC TETRACAINE
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DOI：
10.1021/bi00460a008
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发表时间：
1990-02-27
期刊：
影响因子：
2.9
通讯作者：
WONG, PTT
中科院分区：
文献类型：
--
作者：
AUGER, M;SMITH, ICP;WONG, PTT
高压傅里叶变换红外（FT-IR）光谱用于研究磷脂酰丝氨酸双层的正压行为及其与局麻药丁卡因的相互作用。研究的模型膜系统是 1,2-二肉豆蔻酰-sn-甘油-3-磷酸-L-丝氨酸 (DMPS) 和 1,2-二油酰-sn-甘油-3-磷酸-L-丝氨酸 (DOPS) 的多层水分散体，在 pH 5.5 和 9.5 存在和不存在丁卡因的情况下。红外光谱在28℃下测量。金刚石砧座中的 C 作为高达 25 kbar 压力的函数。结果表明，带负电的磷脂酰丝氨酸双层的正压行为与具有相应酰基链的两性离子磷脂（例如磷脂酰胆碱和磷脂酰乙醇胺）观察到的正压行为非常相似。结果还表明，局麻药在靠近膜-水界面的环境中分配到磷脂酰丝氨酸双层中，并与脂质头基发生静电相互作用。在脂质麻醉系统上应用高静水压力会导致压力诱导麻醉剂从膜排出到水环境中。不饱和脂质(DOPS)从双层排出麻醉剂所需的压力比饱和脂质(DMPS)高得多(分别为约6kbar和约2kbar)。尽管将麻醉剂掺入 DOPS 双层不会显着影响液晶相中无序酰基链的结构和动态特性，但它会有序化凝胶相中的 DMPS 酰基链。
High-pressure Fourier-transform infrared (FT-IR) spectroscopy was used to study the barotropic behavior of phosphatidylserine bilayers and their interactions with the local anesthetic tetracaine. The model membrane systems studied were multilamellar aqueous dispersions of 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (DMPS) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) in the absence and the presence of tetracaine at pH 5.5 and 9.5. The infrared spectra were measured at 28.degree. C in a diamond anvil cells as a function of pressure up to 25 kbar. The results show that the barotropic behavior of the negatively charged phosphatidylserine bilayers is very similar to that observed for zwitterionic phospholipids, such as phosphatidylcholine and phosphatidylethanolamine, with corresponding acyl chains. The results also indicate that the local anesthetic partitions into phosphatidylserine bilayers in an environment close to the membrane-water interface and interacts electrostatically with the lipid head group. Application of high hydrostatic pressure on the lipid-anesthetic systems results in the pressure-induced expulsion of the anesthetic from a membrane to an aqueous environment. The pressures required for expulsion of anesthetic from bilayers are much higher for the unsaturated lipid (DOPS) than for the saturated lipid (DMPS) (.apprxeq. 6 kbar vs .apprxeq. 2 kbar, respectively). Whereas incorporation of the anesthetic into DOPS bilayers does not affect significantly the structural and dynamic properties of the disordered acyl chains in the liquid-crystalline phase, it orders the DMPS acyl chains in the gel phase.