High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes
High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes
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DOI：
10.1016/j.jchromb.2008.06.001
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发表时间：
2008-07-01
影响因子：
3
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Goto-Inoue, Naoko;Hayasaka, Takahiro;Setou, Mitsutoshi
鞘糖脂是细胞中普遍存在的成分。然而，在分析鞘糖脂的技术方面仍有改进的余地。本文报告了我们的高灵敏度和方便的分析技术，结合质谱成像技术对鞘糖脂进行详细的结构分析。我们能够确定详细的神经酰胺结构;即，鞘氨醇碱和脂肪酸，通过在具有10 pmol GM 1的PVDF膜上进行MS/MS/MS分析，其中通过樱草碱染色仅可见微弱的条带。GM 1的检测限约为1 pmol，低于常规报道中的值（10 pmol）。(C)2008 Elsevier B. V.保留所有权利。
Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. He re we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol). (C) 2008 Elsevier B.V. All rights reserved.