PROTEIN SECRETION IN PSEUDOMONAS-AERUGINOSA - CHARACTERIZATION OF 7 XCP GENES AND PROCESSING OF SECRETORY APPARATUS COMPONENTS BY PREPILIN PEPTIDASE
PROTEIN SECRETION IN PSEUDOMONAS-AERUGINOSA - CHARACTERIZATION OF 7 XCP GENES AND PROCESSING OF SECRETORY APPARATUS COMPONENTS BY PREPILIN PEPTIDASE
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DOI：
10.1111/j.1365-2958.1992.tb01550.x
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发表时间：
1992-05-01
影响因子：
3.6
通讯作者：
TOMMASSEN, J
中科院分区：
文献类型：
--
作者：
BALLY, M;FILLOUX, A;TOMMASSEN, J
xcp 基因是铜绿假单胞菌分泌大多数胞外蛋白所必需的。这些基因的产物对于外蛋白通过信号序列依赖性途径到达周质后穿过外膜的转运至关重要。迄今为止，对三个 xcp 基因的分析表明，这种分泌途径在许多革兰氏阴性细菌中是保守的。此外，xcpA 基因与 pilD 相同，pilD 编码参与菌毛（菌毛）亚基加工的肽酶，表明菌毛生物发生和蛋白质分泌之间存在联系。这里展示了其他七个 xcp 基因的核苷酸序列，指定为 xcpR 至 -X。四个编码的 Xcp 蛋白的 N 末端与 IV 型菌毛的 N 末端相似，表明 XcpA 参与了这些 Xcp 蛋白的加工。这确实可以在体内得到证明。此外，另外两种蛋白 XcpR 和 XcpS 与菌毛组装所需的 PilB 和 PilC 蛋白相似。由于 XcpR 和 PilB 显示典型的核苷酸结合位点，因此 ATP 水解可以为两个系统提供能量。
The xcp genes are required for the secretion of most extracellular proteins by Pseudomonas aeruginosa. The products of these genes are essential for the transport of exoproteins across the outer membrane after they have reached the periplasm via a signal sequence-dependent pathway. To date, analysis of three xcp genes has suggested the conservation of this secretion pathway in many Gram-negative bacteria. Furthermore, the xcpA gene was shown to be identical to pilD, which encodes a peptidase involved in the processing of fimbrial (pili) subunits, suggesting a connection between pili biogenesis and protein secretion. Here the nucleotide sequences of seven other xcp genes, designated xcpR to -X, are presented. The N-termini of four of the encoded Xcp proteins display similarity to the N-termini of type IV pili, suggesting that XcpA is involved in the processing of these Xcp proteins. This could indeed be demonstrated in vivo. Furthermore, two other proteins, XcpR and XcpS, show similarity to the PilB and PilC proteins required for fimbriae assembly. Since XcpR and PilB display a canonical nucleotide-binding site, ATP hydrolysis may provide energy for both systems.