ISOLATION AND CHARACTERIZATION OF PLASMINOGEN ACTIVATORS FROM HYPERPLASTIC AND MALIGNANT PROSTATE TISSUE
ISOLATION AND CHARACTERIZATION OF PLASMINOGEN ACTIVATORS FROM HYPERPLASTIC AND MALIGNANT PROSTATE TISSUE
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DOI：
10.1016/0304-4165(84)90129-6
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
BINDER, BR
中科院分区：
文献类型：
--
作者：
KIRCHHEIMER, J;KOLLER, A;BINDER, BR
通过反硫酸铵梯度增溶、明胶- sepharose层析、Sephadex G-150凝胶过滤、魔豆蛋白a - sepharose层析等步骤纯化人前列腺组织的纤溶酶原激活剂，使其具有明显的均匀性。得到了两种活化剂。主要表现出与人尿高分子量尿激酶相似的物理化学、免疫化学和功能特性。另一个，大约是。20%与组织型纤溶酶原激活剂相关，其纤溶酶原激活剂活性通过添加cnbr -纤维蛋白原片段而增强，其模式与血管型纤溶酶原激活剂相似。然而，合成的低分子量的副硝基苯胺底物，pyroglu - gly - arg - pna和H-D-Ile-Pro-Arg-pNA的分子量和酶活性与血管纤溶酶原激活剂不同，但与高分子量的尿激酶相似。
Plasminogen activators from human prostate tissue were purified to apparent homogeneity by a procedure involving reverse ammonium sulfate gradient solubilization, chromatography on gelatine-Sepharose, gel filtration on Sephadex G-150, and chromatography on concanavalin A-Sepharose as a final step. Two activators were obtained. The predominant one exhibited physicochemical, immunochemical and functional properties indistinguishable from human urinary high MW urokinase. The other one, which amounted to .apprx. 20%, was immunochemically related to tissue type plasminogen activator, and its plasminogen activator activity was enhanced by addition of CNBr-fibrinogen fragments in a similar pattern as the vascular plasminogen activator. The MW, however, and enzymatic activities on the synthetic low MW paranitroanilide substrates, pyro-Glu-Gly-Arg-pNA and H-D-Ile-Pro-Arg-pNA, were different from vascular plasminogen activator but similar to high-MW urinary urokinase.