NMR-STUDY OF EXCHANGE-RATES FOR PROTONS INVOLVED IN SECONDARY AND TERTIARY STRUCTURE OF YEAST TRANSFER-RNAPHE
NMR-STUDY OF EXCHANGE-RATES FOR PROTONS INVOLVED IN SECONDARY AND TERTIARY STRUCTURE OF YEAST TRANSFER-RNAPHE
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DOI：
10.1093/nar/4.10.3599
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发表时间：
1977-01-01
影响因子：
14.9
通讯作者：
REDFIELD, AG
中科院分区：
文献类型：
--
作者：
JOHNSTON, PD;REDFIELD, AG
通过饱和恢复长脉冲傅立叶变换 NMR 测量了低场 NMR 区域（DSS 的−11 至 -15 ppm）中酵母 tRNA 球体共振的所有质子的溶剂交换率。酵母 tRNAphe 中的所有这些质子相对于其内在的纵向弛豫过程都处于与 H2O 的快速交换极限。大多数速率表现出很小的温度依赖性；然而，在较高温度下，在不存在 Mg+4 的情况下，叔碱基对质子优先不稳定。对于 10°C 至 45°C 的温度范围以及 0 至 15 mM 的 MgCl2 浓度，测得的交换速率在 2 至 125 sec−1 之间。
Solvent exchange rates of all the protons of yeast tRNApheresonating in the lowfield NMR region (−11 to −15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNApheare in the fast exchange limit with H2O relative to their intrinsic longitudinal relaxation processes. Most rates show very little temperature dependence; however, tertiary base pairprotons are preferentially destabilized in the absence of Mg+4at higher temperatures. The measured exchange rates are between 2 and 125 sec−1for a temperature range from 10°C to 45°C and MgC12concentrations between 0 and 15 mM.