Role of azithromycin against clinical isolates of family enterobacteriaceae: A comparison of its minimum inhibitory concentration by three different methods
Role of azithromycin against clinical isolates of family enterobacteriaceae: A comparison of its minimum inhibitory concentration by three different methods
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DOI：
10.4103/0255-0857.45361
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发表时间：
2009-04-01
影响因子：
1.6
通讯作者：
Das, P.
中科院分区：
文献类型：
--
作者：
Chayani, N.;Tiwari, S.;Das, P.
目的：目的观察新型氮杂内酯类抗生素阿奇霉素对肠杆菌科临床分离株的作用，并采用纸片扩散法、琼脂稀释法和E-test法测定其最低抑菌浓度（MIC），并进行比较。材料与方法：采用纸片扩散法、琼脂稀释法和E-test法对临床分离的159株肠杆菌科细菌进行阿奇霉素敏感性试验。分析了MIC值，并提到了不同方法之间的一致性百分比。结果：159株肠杆菌科细菌中，大肠杆菌60.37株;大肠杆菌属次之，克雷伯氏菌属28.3株，沙门氏菌和志贺氏菌属3.77株，肠杆菌和柠檬酸杆菌属各1.88株。从尿液117/159（73.58）中获得最大分离株。阿奇霉素对沙门氏菌和志贺氏菌的敏感性较高，E-试验敏感性为100，纸片扩散法敏感性为83.33。在琼脂稀释法中，83.33的沙门菌和66.66的志贺菌对阿奇霉素敏感。纸片扩散法与琼脂稀释法的总体一致性为96.8，琼脂稀释法与E-test的总体一致性为88，纸片扩散法与E-test的总体一致性为91.2。结论：阿奇霉素可能成为我们抗菌策略的重要补充，特别是用于治疗细菌性腹泻和伤寒沙门氏菌引起的感染。
Purpose: To determine the effect of azithromycin, a new azalide antibiotic, on clinical isolates of the family Enterobacteriaceae and to determine and compare its minimum inhibitory concentration (MIC) by disk diffusion, agar dilution and E-test methods. Materials and Methods: One hundred fifty-nine bacterial strains belonging to the family Enterobacteriaceae, isolated from different clinical samples, were tested for their susceptibility to azithromycin by disk diffusion, agar dilution and E-test methods. The MIC values were analysed and the percentages of agreement between the different methods were mentioned. Results: Of the 159 isolates of the family Enterobacteriaceae, 60.37 were E. coli followed by Klebsiella species 28.3, Salmonella and Shigella species 3.77 and Enterobacter and Citrobacter species 1.88 each. Maximum isolates were obtained from urine 117/159 (73.58). Azithromycin was found to be more active against Salmonella and Shigella species, showing 100 sensitivity the by E-test and 83.33 by the disk diffusion methods. In the agar dilution method, 83.33 of Salmonella and 66.66 of Shigella species were sensitive to azithromycin. The overall agreement between disk diffusion and agar dilution method was 96.8, between agar dilution and E-test was 88 and between disk diffusion and E-test was 91.2. Conclusion: Azithromycin may become an important addition to our antimicrobial strategies, especially for the treatment of bacterial diarrhoea and infections caused by Salmonella typhi.