Characterization of the CP complex, an abundant dimer of Cdc68 and Pob3 proteins that regulates yeast transcriptional activation and chromatin repression
Characterization of the CP complex, an abundant dimer of Cdc68 and Pob3 proteins that regulates yeast transcriptional activation and chromatin repression
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DOI：
10.1074/jbc.273.34.21972
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发表时间：
1998-08-21
影响因子：
4.8
通讯作者：
Singer, RA
中科院分区：
文献类型：
--
作者：
Brewster, NK;Johnston, GC;Singer, RA
酵母酿酒酵母的Cdc 68蛋白是一种必需的核蛋白，已显示其对于许多基因的反式激活以及对于在不存在反式激活的情况下维持染色质介导的阻遏是必需的。这些活性暗示Cdc 68蛋白在染色质结构和/或功能的调节中，在这里，我们报告说，Cdc 68被发现与另一种必需的核蛋白，Pob 3，在我们所谓的CP复合物。这种Cdc 68与Pob 3的二聚体对于部分纯化是稳定的，因此分配给Cdc 68的基因激活和抑制的功能可能是CP复合物的性质。CP复合物是非常丰富的，这表明它可能是广泛分布在整个染色质。
The Cdc68 protein of the yeast Saccharomyces cerevisiae is an essential nuclear protein that has been shown to be necessary for the tl-ans-activation of many genes as well as for the maintenance of chromatin-mediated repression in the absence of trans-activation, These activities implicate, the Cdc68 protein in the regulation of chromatin structure and/or function, Here we report that Cdc68 is found in association with another essential nuclear protein, Pob3, in what we term the CP complex. This dimer of Cdc68 with Pob3 is stable to partial purification, so that the functions of gene activation and repression that are assigned to Cdc68 are likely to be properties of the CP complex. The CP complex is highly abundant, suggesting that it may be widespread throughout chromatin.