Effect of Lichong decoction on expression of Bcl-2 and Bcl-2-associated X protein mRNAs in hysteromyoma model rat
Effect of Lichong decoction on expression of Bcl-2 and Bcl-2-associated X protein mRNAs in hysteromyoma model rat
复制标题
理冲汤对子宫肌瘤模型大鼠Bcl-2及Bcl-2相关X蛋白mRNA表达的影响
DOI：
10.1016/s0254-6272(13)60132-6
复制
发表时间：
2013-04-15
影响因子：
2.6
通讯作者：
Zou, Xiaoli
中科院分区：
文献类型：
--
作者：
Li, Donghua;Xu, Xin;Zou, Xiaoli
目的：探讨理冲汤对子宫肌瘤模型大鼠子宫肌瘤组织中凋亡调控基因Bcl-2及Bcl-2相关X蛋白（Box）mRNA表达的影响。方法：将50只Wistar雌性大鼠随机分为正常组、模型组、理冲汤组、桂枝茯苓胶囊组和米非司酮组。腹腔注射外源性雌激素和孕激素建立大鼠子宫肌瘤模型。光学显微镜下观察子宫组织病理学改变、子宫系数及子宫横径，实时荧光定量聚合酶链反应（PCR）技术检测各组子宫组织Bcl-2和Bax mRNA的表达。治疗后，光镜下发现理冲汤组子宫肌层变薄，肌纤维轻度增生或细长，理冲汤组内环肌、外纵肌排列整齐，偶见或未见插入现象。子宫系数和子宫横径明显减小（P
OBJECTIVE: To study on effects of Lichong decoction on expression of apoptosis-controlling genes, Bcl-2 and Bcl-2-associated X protein (Box) mRNAs in hysteromyoma tissue of the hysteromyoma model rat.METHODS: Fifty Wistar female rats were randomly divided into a normal group, a model group, a Lichong decoction group, a Guizifuling capsule group and a Mifepristone group. The hysteromyoma rat model was established by intraperitoneal injection of exogenous estrin and progestogens. Pathological examination of uterine tissue, uterine coefficient and uterine transverse diameter were made under optic microscope and expressions of Bcl-2 and Bax mRNAs in uterine tissue in the groups were detected with real-time fluorescent quantitative polymerase chain reaction (PCR) technique.RESULTS: After treatment, under microscope it was found that in the Lichong decoction group myometrium thinned, muscle fiber slightly overgrowth or long and thin, regular arrangement, inserting phenomenon of inner circular muscle and external longitudinal muscle was occasionally or not seen in the Lichong decoction group. The uterine coefficient and the uterine transverse diameter significantly decreased (P