The Defense Metabolite, Allyl Glucosinolate, Modulates Arabidopsis thaliana Biomass Dependent upon the Endogenous Glucosinolate Pathway.
The Defense Metabolite, Allyl Glucosinolate, Modulates Arabidopsis thaliana Biomass Dependent upon the Endogenous Glucosinolate Pathway.
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DOI：
10.3389/fpls.2016.00774
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发表时间：
2016
影响因子：
5.6
通讯作者：
Kliebenstein DJ
中科院分区：
文献类型：
--
作者：
Francisco M;Joseph B;Caligagan H;Li B;Corwin JA;Lin C;Kerwin R;Burow M;Kliebenstein DJ
硫代葡萄糖苷（GSL）作为生物和非生物胁迫反应的直接介质在植物中发挥着重要作用。最近的研究开始表明，GSL 还可以诱导复杂的防御和增长网络。然而，这些 GSL 诱导的反应的生理意义以及 GSL 被感知和/或调节这些反应的分子机制尚不清楚。为了确定植物内的这些潜在机制以及它们如何与内源 GSL 相关，我们测试了外源烯丙基 GSL 应用对拟南芥种质样品的生长和防御代谢的调节作用。我们发现，应用外源烯丙基 GSL 能够引发植物生物量的变化和防御代谢物的积累，这些代谢物在不同种质中存在遗传差异。这种生长效应与烯丙基GSL侧链结构有关。利用 GSL 途径内基因的这种自然变异和突变体，我们可以证明烯丙基 GSL 和改变的生长反应之间的联系取决于控制脂肪族 GSL 途径的已知基因的功能。
Glucosinolates (GSLs) play an important role in plants as direct mediators of biotic and abiotic stress responses. Recent work is beginning to show that the GSLs can also inducing complex defense and growth networks. However, the physiological significance of these GSL-induced responses and the molecular mechanisms by which GSLs are sensed and/or modulate these responses are not understood. To identify these potential mechanisms within the plant and how they may relate to the endogenous GSLs, we tested the regulatory effect of exogenous allyl GSL application on growth and defense metabolism across sample of Arabidopsis thaliana accessions. We found that application of exogenous allyl GSL had the ability to initiate changes in plant biomass and accumulation of defense metabolites that genetically varied across accessions. This growth effect was related to the allyl GSL side-chain structure. Utilizing this natural variation and mutants in genes within the GSL pathway we could show that the link between allyl GSL and altered growth responses are dependent upon the function of known genes controlling the aliphatic GSL pathway.