Nanoscale Aggregation of Cellular β2-Adrenergic Receptors Measured by Plasmonic Interactions of Functionalized Nanoparticles
Nanoscale Aggregation of Cellular β2-Adrenergic Receptors Measured by Plasmonic Interactions of Functionalized Nanoparticles
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DOI：
10.1021/nn900488u
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发表时间：
2009-08-01
期刊：
影响因子：
17.1
通讯作者：
Pezacki, John Paul
中科院分区：
文献类型：
--
作者：
Kennedy, David C.;Tay, Li-Lin;Pezacki, John Paul
控制心肌细胞收缩和哺乳动物心脏跳动的肾上腺素能信号是通过与细胞膜上纳米级多蛋白复合物中的肾上腺素能受体结合的配体启动的。在这里，我们证明了功能化银纳米粒子的表面增强拉曼散射（SERS）可以用来报告小鼠心肌细胞上特异性标记β(2)-肾上腺素能受体的受体聚集状态。此外，包括拉曼、瑞利散射、扫描电镜和发光成像在内的多模态成像相结合，充分表征了β(2)-肾上腺素能受体介导的银纳米颗粒在心肌细胞膜上的聚集。扫描电镜分析显示，细胞膜上β(2)-肾上腺素能受体簇的超高分辨率图像显示，每个信号域有10到70个纳米粒子的明显SERS活性簇。这些技术可以普遍应用于研究其他细胞表面受体的聚集和探索它们在细胞表面的分布。
Adrenergic signaling that controls the contraction of cardiac myocyte cells and the beating of the mammalian heart is initiated by ligand binding to adrenergic receptors contained in nanoscale multiprotein complexes at the cellular membrane. Here we demonstrate that the surface-enhanced Raman scattering (SERS) of functionalized silver nanoparticles can be used to report on the receptor aggregation state of specifically label beta(2)-adrenergic receptors on mouse cardiac myocyte cells. Furthermore, multimodal imaging including Raman, Rayleigh scattering, scanning electron microscopy, and luminescence imaging was combined to fully characterize the beta(2)-adrenergic receptor-mediated aggregation of silver nanoparticles on the membrane of cardiac myocytes. Scanning electron microscopy analysis reveals distinct SERS active clusters of between 10 and 70 nanoparticles per signaling domain from ultra-high-resolution images Of beta(2)-adrenergic receptor clusters on the cellular membrane. These techniques can be generally applied to study the aggregation of other cell surface receptors and explore their distribution on cell surfaces.