Ectopic decapentaplegic in the Drosophila midgut alters the expression of five homeotic genes, dpp, and wingless, causing specific morphological defects.
Ectopic decapentaplegic in the Drosophila midgut alters the expression of five homeotic genes, dpp, and wingless, causing specific morphological defects.
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DOI：
10.1006/dbio.1994.1219
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发表时间：
1994-08
影响因子：
2.7
通讯作者：
K. Staehling‐Hampton;F. Hoffmann
中科院分区：
文献类型：
--
作者：
K. Staehling‐Hampton;F. Hoffmann
果蝇中肠内脏中胚层中的同源基因表达模式在多种形态发生事件中发挥重要作用，包括胃盲肠的形成和三个中肠收缩的定位。我们证明，内脏中胚层同源异型基因表达的有效调节剂是由十肢麻痹（dpp）基因编码的分泌性生长因子样分子。内脏中胚层中的异位 dpp 引起性梳、触角足、超胸 (Ubx) 和腹部 A (abd-A) 基因表达的变化，并破坏胃盲肠和第一和第三中肠收缩的形成。异位 dpp 还诱导内脏中胚层中 teashirt、wingless (wg) 和内源 dpp 基因的表达，并增强邻近内胚层中唇部的表达。在异位 dpp 存在的情况下，基因表达模式和第二中肠缢缩的形成与 dpp 诱导的几乎整个中肠向细胞命运的转化最为一致，通常仅在中肠的副段 (ps)7 和 ps8 区域中看到。我们得出的结论是，dpp 是维持内脏中胚层中 Ubx 表达的主要信号，其模式与胚胎外胚层中的 Ubx 表达不同，并提供细胞间通讯机制，通过该机制，Ubx 表达影响内脏中胚层中跨胚层和跨 ps7 至 ps8 副段边界的基因表达。
The patterns of homeotic gene expression in the Drosophila midgut visceral mesoderm are instrumental in several morphogenetic events, including the formation of the gastric caeca and the positioning of the three midgut constrictions. We demonstrate that a potent regulator of homeotic gene expression in the visceral mesoderm is the secreted growth factor-like molecule encoded by the decapentaplegic (dpp) gene. Ectopic dpp in the visceral mesoderm caused changes in the gene expression of Sex combs reduced, Antennapedia, Ultrabithorax (Ubx), and abdominal-A (abd-A) and disrupted the formation of the gastric caeca and the first and third midgut constrictions. Ectopic dpp also induced expression of teashirt, wingless (wg) and the endogenous dpp gene in the visceral mesoderm and enhanced labial expression in the adjacent endoderm. The patterns of gene expression and the formation of the second midgut constriction in the presence of ectopic dpp are most consistent with a dpp-induced transformation of virtually the entire midgut to cell fates normally seen only in the parasegment (ps)7 and ps8 regions of the midgut. We conclude that dpp is a primary signal in maintaining Ubx expression in the visceral mesoderm in a pattern different from Ubx expression in the embryonic ectoderm and in providing a cell-cell communication mechanism by which Ubx expression influences gene expression across germlayers and across the ps7 to ps8 parasegment boundary in the visceral mesoderm.