Detecting and quantifying influenza virus with self- versus investigator-collected mid-turbinate nasal swabs
Detecting and quantifying influenza virus with self- versus investigator-collected mid-turbinate nasal swabs
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DOI：
10.1002/jmv.24753
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发表时间：
2017-07-01
影响因子：
12.7
通讯作者：
Kwong, Jeffrey C.
中科院分区：
文献类型：
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作者：
Granados, Andrea;Quach, Susan;Kwong, Jeffrey C.
我们比较了自己和研究人员收集的鼻甲中拭子来检测和量化流感病毒载量。我们使用核糖核酸酶P，它反映了人类细胞的存在，以确定足够的样本收集。共采集流感阳性拭子16对，流感阴性拭子25对。自行采集和研究人员采集的拭子甲型流感病毒载量中位数分别为1.68和1.67log10拷贝/毫升(P=0.96)。自己采集的拭子和调查人员采集的拭子之间的RNaseP载量也相似(P=0.51)。自我收集的鼻甲中拭子产生的病毒载量与研究人员收集的拭子相当，因此可以考虑用于研究和临床治疗。
We compared pairs of self- and investigator-collected mid-turbinate nasal swabs to detect and quantify influenza viral loads. We used RNase P, which reflects presence of human cells to determine adequate sample collection. Sixteen pairs of influenza-positive swabs and 25 pairs of influenza-negative swabs were included in this study. The median influenza A viral loads for self- and investigator-collected swabs were 1.68 and 1.67 log10 copies/mL, respectively (P = 0.96). RNase P loads were also similar between self-and investigator-collected swabs (P = 0.51). Self-collected mid-turbinate nasal swabs yield comparable viral loads to investigator-collected swabs, and therefore might be considered for research and clinical management.