Rice gene OsNAC19 encodes a novel NAC-domain transcription factor and responds to infection by Magnaporthe grisea
Rice gene OsNAC19 encodes a novel NAC-domain transcription factor and responds to infection by Magnaporthe grisea
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DOI：
10.1016/j.plantsci.2006.07.019
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发表时间：
2007-01-01
期刊：
影响因子：
5.2
通讯作者：
Peng, Youliang
中科院分区：
文献类型：
--
作者：
Lin, Ruiming;Zhao, Wensheng;Peng, Youliang
植物特异的nac结构域蛋白在植物的逆境响应和发育调控中起着重要的作用。本研究从稻瘟病菌（Magnaporthe grisea） 131杂交种侵染水稻叶片制备的cDNA文库中，分离到一个编码新nac结构域蛋白的全长cDNA克隆OsNAC19 （Oryza sativa NAC19）。OsNAC 19与OsNAC3亚群成员的相似度为65.87 ~ 51.53%。一个植物特异性的NAC结构域位于OsNAC19的n端。OsNAC19蛋白定位于洋葱流行细胞的细胞核中。在酵母检测系统中，OsNAC19与GAL4 dna结合域（BD）的融合蛋白激活了报告基因LacZ和HIS3，激活区域位于其c端区181-240氨基酸。OsNAC19基因在水稻幼苗根、茎和叶鞘中表达量高，在水稻叶片中表达量低。稻瘟病菌侵染、外源茉莉酸甲酯（MeJA）、ABA和乙烯均能诱导OsNAC19在水稻叶片中的表达，但乙烯的诱导作用相对较弱。这些数据表明OsNAC19蛋白是一个转录激活因子，参与水稻对稻瘟病菌感染的应答，并可能在meja介导的信号通路中发挥作用。2006爱思唯尔爱尔兰有限公司版权所有。
The plant-specific NAC-domain proteins have been identified to play important roles in plant responses to stresses or in plant development regulation. In this research, a full-length cDNA clone OsNAC19 (Oryza sativa NAC19), encoding a novel NAC-domain protein, was isolated from a cDNA library prepared with rice leaves infected by incompatible race 131 of blast fungus (Magnaporthe grisea). The similarity between OsNAC 19 and the members of OsNAC3 subgroup is from 65.87 to 51.53%. One plant-specific NAC domain is located in the N-terminus of OsNAC19. The protein OsNAC19 was localized in the nucleus of onion epidemical cells. The fusion protein of OsNAC19 with GAL4 DNA-binding domain (BD) activated the reporter genes LacZ and HIS3 in a yeast assay system, and the activation domain was located within amino acid 181-240 of its C-terminal region. The expression of gene OsNAC19 was high in rice seedling roots, culms and blade sheathes, but its expression in rice leaves was low. The expression of OsNAC19 in rice leaves could be induced by the infection of blast fungus, and by application of exogenous methyl jasmonate (MeJA), ABA and ethylene but ethylene had a relatively weak induction effect. These data suggest that OsNAC19 protein is a transcriptional activator involved in rice response to infection by M. grisea and may play a role in the MeJA-mediated signaling pathway. (c) 2006 Elsevier Ireland Ltd. All rights reserved.