Mucin gene expression in human middle ear epithelium
Mucin gene expression in human middle ear epithelium
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DOI：
10.1097/mlg.0b013e31806db531
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发表时间：
2007-09-01
期刊：
影响因子：
2.6
通讯作者：
Kerschner, Joseph Edward
中科院分区：
文献类型：
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作者：
Kerschner, Joseph Edward
目的：研究最近鉴定的人粘蛋白基因在人中耳上皮（MEE）标本中的表达，并比较这种粘蛋白基因。方法：收集人胰岛细胞作为体内标本，建立人胰岛细胞培养物用于体外实验。从大肠杆菌中提取RNA，并设计用于逆转录聚合酶链反应的引物，以评估粘蛋白基因MUC1、MUC2、MUC3、MUC4、MUC5AC、MUC5B、MUC6、MUC7、MUC8、MUC9、MUC11、MUC12、MUC13、MUC15、MUC16、MUC18、MUC19和MUC20的表达。结果：粘蛋白基因MUC1、MUC2、MUC3、MUC4、MUC5AC、MUC5B、MUC7、MUC8、MUC9、MUC11、MUC13、MUC15、MUC16、MUC18、MUC19和MUC20在体内和体外ME组织中均有表达。粘蛋白基因MUC6、MUC12和MUC17在两种组织样品中均未被鉴定。结论：许多最近被鉴定的粘蛋白基因在人乳腺癌中表达。这些基因在体内和体外模型中以相似的方式表达。了解这些基因调节中耳炎的生理学和病理生理学的机制，将提供对中耳炎和中耳炎等疾病的分子机制的更透彻的理解。
Objectives: To investigate the expression of recently identified human mucin genes in human middle ear epithelial (MEE) specimens from in vivo middle ear (ME) tissue and to compare this mucin gene. expression with mucin gene expression in an immortalized cell culture in vitro source of human MEE.Methods: Human MEE was harvested as in vivo specimens, and human MEE cell cultures were established for in vitro experimentation. RNA was extracted from MEE and primers designed for reverse-transcription polymerase chain reaction to assess for mucin gene MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC6, MUC7, MUC8, MUC9, MUC11, MUC12, MUC13, MUC15, MUC16, MUC18, MUC19, and MUC20 expression. Mucin gene expression in the in vivo and in vitro ME tissue was compared against tissues with known expression of the mucin genes in question.Results: Mucin genes MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC7, MUC8, MUC9, MUC11, MUC13, MUC15, MUC16, MUC18, MUC19, and MUC20 were identified and expressed in both the in vivo and in vitro samples of MEE. Mucin genes MUC6, MUC12, and MUC17 were not identified in either tissue samples.Conclusion: Many of the mucin genes that have been recently identified are expressed in human MEE. These genes are expressed in a similar manner in both in vivo and in vitro models. Understanding the mechanisms in which these genes regulate the physiology and pathophysiology of MEE will provide a more thorough understanding of the molecular mechanics of the MEE and disease conditions such as otitis media.