The use of monoclonal antibody to keratin in human epidermal disease: alterations in immunohistochemical staining pattern.
The use of monoclonal antibody to keratin in human epidermal disease: alterations in immunohistochemical staining pattern.
复制标题
角蛋白单克隆抗体在人类表皮疾病中的应用：免疫组织化学染色模式的改变。
DOI：
10.1111/1523-1747.ep12518198
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Sun,TT
中科院分区：
文献类型：
--
作者：
Weiss,RA;Guillet,GY;Freedberg,IM;Farmer,ER;Small,EA;Weiss,MM;Sun,TT
用一种抗角蛋白的单抗AE1，用免疫荧光和过氧化物酶-抗过氧化物酶技术对正常和异常皮肤冰冻切片进行染色。在正常人表皮和寻常型鱼鳞病(一种非增殖性表皮疾病)中，该抗体选择性地对表皮基底细胞进行染色。在其他各种表皮疾病中也观察到了非常不同的染色模式。银屑病(16例)、疣(9例)、脂溢性角化病(5例)、光化性角化病(2例)以及与某些表皮肿瘤相邻的表皮(4例)均呈基础以上染色。基底细胞癌(7例)呈弱均匀染色。相反，在鲍温氏病(2例)和鳞状细胞癌(4例)中观察到由不同染色强度的细胞组成的无序图案。虽然这些改变的染色模式的生化基础仍有待阐明，但这些结果提供了进一步的证据，表明表皮角蛋白的表达可以受到不同疾病状态的影响。此外，我们的数据表明，在牛皮癣和其他一些良性的增生性表皮疾病中，角蛋白表达的共同变化，如基础以上AE1染色模式所定义的那样。
A monoclonal antikeratin antibody, designated AE1, was used to stain frozen sections of normal and abnormal human skin by the immunofluorescence and peroxidase-antiperoxidase techniques. In normal human epidermis and ichthyosis vulgaris, a nonproliferative epidermal disease, this antibody selectively stained epidermal basal cells. Very different staining patterns were observed in various other epidermal diseases. A suprabasal staining pattern was observed in psoriasis (16 cases), verruca (9), seborrheic keratosis (5), actinic keratosis (2), as well as the epidermis adjacent to certain epidermal neoplasms (4). Basal cell carcinoma (7) showed weak, homogeneous staining. In contrast, a disorganized pattern consisting of cells with various staining intensities was observed in Bowen's disease (2) and squamous cell carcinoma (4). Although the biochemical basis for these altered staining patterns remains to be elucidated, these results provide further evidence that epidermal keratin expression can be affected by various disease states. Moreover, our data suggest that a common alteration in keratin expression, as defined by the suprabasal AE1 staining pattern, exists in psoriasis and a number of other benign hyperproliferative epidermal diseases.