Characterization of the Conus bullatus genome and its venom-duct transcriptome.
Characterization of the Conus bullatus genome and its venom-duct transcriptome.
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DOI：
10.1186/1471-2164-12-60
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发表时间：
2011-01-25
期刊：
影响因子：
4.4
通讯作者：
Yandell M
中科院分区：
文献类型：
--
作者：
Hu H;Bandyopadhyay PK;Olivera BM;Yandell M
有毒的海洋腹足类动物，锥螺（属），注射猎物与致命的鸡尾酒锥肽，小的富含半胱氨酸的肽，每一个具有高亲和力的分子目标，通常是一个离子通道，受体或转运。在过去的十年中，锥肽已被证明是研究脊椎动物神经传递不可或缺的试剂。泡状芋螺属于芋螺属的一个分支，称为Textilia，其药理学特征仍然很差。因此，这里提出的基因组学分析提供了第一步，更好地了解神秘的纺织分支。我们已经进行了大泡芋螺基因组和毒管转录组的测序调查。我们发现，conopeptides是高度表达的毒液导管内，并描述了在计算机管道中使用RNA-seq数据的发现和表征。我们还进行了基因组的低覆盖率鸟枪测序，并使用这些数据来确定其大小，全基因组碱基组成，简单重复序列和移动的元件密度。我们的结果首次提供了任何锥形蜗牛中毒管转录的整体视图。大泡芋螺毒液的一个显著特征是在毒液导管中表达的A-超家族肽的宽度，其结构多样性是前所未有的。我们还发现，与C的其余部分相比，锥肽内的SNP率更高。bullatus转录组，与锥肽在多样化选择下的假设一致。
The venomous marine gastropods, cone snails (genus Conus), inject prey with a lethal cocktail of conopeptides, small cysteine-rich peptides, each with a high affinity for its molecular target, generally an ion channel, receptor or transporter. Over the last decade, conopeptides have proven indispensable reagents for the study of vertebrate neurotransmission. Conus bullatus belongs to a clade of Conus species called Textilia, whose pharmacology is still poorly characterized. Thus the genomics analyses presented here provide the first step toward a better understanding the enigmatic Textilia clade. We have carried out a sequencing survey of the Conus bullatus genome and venom-duct transcriptome. We find that conopeptides are highly expressed within the venom-duct, and describe an in silico pipeline for their discovery and characterization using RNA-seq data. We have also carried out low-coverage shotgun sequencing of the genome, and have used these data to determine its size, genome-wide base composition, simple repeat, and mobile element densities. Our results provide the first global view of venom-duct transcription in any cone snail. A notable feature of Conus bullatus venoms is the breadth of A-superfamily peptides expressed in the venom duct, which are unprecedented in their structural diversity. We also find SNP rates within conopeptides are higher compared to the remainder of C. bullatus transcriptome, consistent with the hypothesis that conopeptides are under diversifying selection.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
2.8
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DOI：
10.1073/pnas.2335845100
发表时间：
2003-11-25
影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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