Lipid correction for carbon stable isotope analysis of deep-sea fishes
Lipid correction for carbon stable isotope analysis of deep-sea fishes
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DOI：
10.1016/j.dsr.2010.05.003
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发表时间：
2010-08-01
影响因子：
2.4
通讯作者：
Sutton, Tracey T.
中科院分区：
文献类型：
--
作者：
Hoffman, Joel C.;Sutton, Tracey T.
鱼类组织的稳定同位素分析有助于深海食物网的研究，因为取样困难严重限制了传统饮食研究的鱼类样本量。碳稳定同位素比率（Δ C-13）被广泛用于食物网研究，但它必须加以校正，以消除与组织中不同脂质含量相关的变异性。尚未确定任何深海鱼类的脂质校正。这些鱼类是研究脂质校正的理想选择，因为不同物种的脂质含量差异很大。我们的目的是评估应用程序的质量平衡三角洲C-13校正的分类上不同的组的深海鱼类的稳定同位素比值的影响，确定脂质提取，检查质量的模型参数推导出的质量平衡校正，并比较校正发表的结果。我们测量了从30个北大西洋物种的肌肉组织的氮稳定同位素比率（Δ N-15）和Δ C-13的脂质提取效果。脂质提取显著增加了组织Δ N-15（+0.66 ‰）和Δ C-13值，但对Δ C-13的治疗效果取决于C：N，这是脂质含量的代表。我们将脂质提取的Δ C-13与通过质量平衡校正预测的Δ C-13进行了比较，所述质量平衡校正使用从所有个体（合并）或逐个物种估计的模型变量或使用来自其他物种的公布值。使用逐种方法进行的校正效果最好;然而，所有三种方法产生的校正值通常在测量的无脂质Δ C-13的0.5 ppm范围内，并且具有较小的总体偏差（< 0.5 ppm）。我们得出结论，广义质量平衡校正适用于校正深海鱼类中的δ C-13，类似于为其他鱼类开发的校正，并建议在对高脂质含量的鱼类（C：N > 8）应用广义校正时应谨慎。爱思唯尔有限公司出版
Stable isotope analysis of fish tissue can aid studies of deep-sea food webs because sampling difficulties severely limit sample sizes of fish for traditional diet studies. The carbon stable isotope ratio (delta C-13) is widely used in food web studies, but it must be corrected to remove variability associated with varying lipid content in the tissue. A lipid correction has not been determined for any deep-sea fish. These fishes are ideal for studying lipid correction because lipid content varies widely among species. Our objective was to evaluate an application of a mass balance delta C-13 correction to a taxonomically diverse group of deep-sea fishes by determining the effect of lipid extraction on the stable isotope ratios, examining the quality of the model parameters derived for the mass balance correction, and comparing the correction to published results. We measured the lipid extraction effect on the nitrogen stable isotope ratio (delta N-15) and delta C-13 of muscle tissue from 30 North Atlantic species. Lipid extraction significantly increased tissue delta N-15 ( +0.66 parts per thousand) and delta C-13 values, but the treatment effect on delta C-13 was dependent on C:N, a proxy for lipid content. We compared the lipid-extracted delta C-13 to the delta C-13 predicted by the mass balance correction using model variables estimated from either all individuals (pooled) or species-by-species or using published values from other species. The correction using the species-by-species approach performed best; however, all three approaches produced corrected values that were generally within 0.5 parts per thousand of the measured lipid-free delta C-13 and that had a small over-all bias ( < 0.5 parts per thousand). We conclude that a generalized mass balance correction works well for correcting delta C-13 in deep-sea fishes, is similar to that developed for other fishes, and recommend caution when applying a generalized correction to fish with high lipid content (C:N > 8). Published by Elsevier Ltd.