Genomic Analysis Reveals Multi-Drug Resistance Clusters in Group B Streptococcus CC17 Hypervirulent Isolates Causing Neonatal Invasive Disease in Southern Mainland China.
Genomic Analysis Reveals Multi-Drug Resistance Clusters in Group B Streptococcus CC17 Hypervirulent Isolates Causing Neonatal Invasive Disease in Southern Mainland China.
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DOI：
10.3389/fmicb.2016.01265
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发表时间：
2016
影响因子：
5.2
通讯作者：
Rinaudo CD
中科院分区：
文献类型：
--
作者：
Campisi E;Rosini R;Ji W;Guidotti S;Rojas-López M;Geng G;Deng Q;Zhong H;Wang W;Liu H;Nan C;Margarit I;Rinaudo CD
B 族链球菌 (GBS) 引起的新生儿侵袭性疾病是全球重大的公共卫生保健问题。然而，中国和其他亚洲国家有关疾病负担、血清型分布和分子流行病学的数据很少，而且特别是与有限区域有关。本研究的目的是调查 2013 年至 2014 年中国南方广州和长沙医院从患有侵袭性疾病的新生儿中回收的 GBS 分离株的遗传特征。我们评估了 26 个新生儿临床分离株的荚膜多糖类型、菌毛岛 (PI) 分布和 hvgA 基因的存在，其中 8 个是从早发性疾病中恢复的，18 个是从晚发性疾病 (LOD) 中恢复的。在检查的 26 个分离株中，鉴定出 5 种血清型。 III 型最为常见（15 例），特别是在 LOD 菌株中（n = 11），其次是 Ib 型（n = 5）、V 型（n = 3）、Ia 型（n = 2）和 II 型（n = 1）。我们对属于高毒力克隆复合物 17（血清型 III-CC17）的 14 个血清型 III 分离株进行了全基因组测序分析和抗菌药物敏感性测试。 PI-2b 单独存在与 14 种血清型 III-CC17 菌株中的 13 种相关。基因组分析使我们确定了两个新版本的整合和接合元件（ICE）中包含的两个多重耐药基因簇，分别携带五个或八个抗生素抗性基因。这些 ICE 取代了通常包含 PI-1 操纵子的 16 kb 基因座。所有含有已鉴定的 ICE 的分离株均表现出对氨基糖苷类、大环内酯类和四环素类抗生素的多重耐药性。总之，我们报告了对中国分离的 14 种 GBS 血清型 III-CC17 菌株的首次全基因组序列分析，这些菌株代表了引起新生儿侵袭性疾病的最常见谱系。新发现的具有多种抗生素耐药性的 ICE 的获得可以部分解释这种特定克隆在中国新生儿分离株中的传播，并强调持续流行病学监测的必要性。
Neonatal invasive disease caused by group B Streptococcus (GBS) represents a significant public health care concern globally. However, data related to disease burden, serotype distribution, and molecular epidemiology in China and other Asian countries are very few and specifically relative to confined regions. The aim of this study was to investigate the genetic characteristics of GBS isolates recovered from neonates with invasive disease during 2013–2014 at Guangzhou and Changsha hospitals in southern mainland China. We assessed the capsular polysaccharide type, pilus islands (PIs) distribution and hvgA gene presence in a panel of 26 neonatal clinical isolates, of which 8 were recovered from Early Onset Disease and 18 from Late Onset Disease (LOD). Among 26 isolates examined, five serotypes were identified. Type III was the most represented (15 cases), particularly among LOD strains (n = 11), followed by types Ib (n = 5), V (n = 3), Ia (n = 2) and II (n = 1). We performed whole-genome sequencing analysis and antimicrobial susceptibility testing on the 14 serotype III isolates belonging to the hypervirulent Clonal Complex 17 (serotype III-CC17). The presence of PI-2b alone was associated with 13 out of 14 serotype III-CC17 strains. Genome analysis led us to identify two multi-drug resistance gene clusters harbored in two new versions of integrative and conjugative elements (ICEs), carrying five or eight antibiotic resistance genes, respectively. These ICEs replaced the 16 kb-locus that normally contains the PI-1 operon. All isolates harboring the identified ICEs showed multiple resistances to aminoglycoside, macrolide, and tetracycline antibiotic classes. In conclusion, we report the first whole-genome sequence analysis of 14 GBS serotype III-CC17 strains isolated in China, representing the most prevalent lineage causing neonatal invasive disease. The acquisition of newly identified ICEs conferring multiple antibiotic resistance could in part explain the spread of this specific clone among Chinese neonatal isolates and underlines the need for a constant epidemiological surveillance.