HUMAN-ANTIBODY RESPONSE TO PLASMODIUM-FALCIPARUM MEROZOITE SURFACE PROTEIN-2 IS SEROGROUP SPECIFIC AND PREDOMINANTLY OF THE IMMUNOGLOBULIN G3 SUBCLASS
HUMAN-ANTIBODY RESPONSE TO PLASMODIUM-FALCIPARUM MEROZOITE SURFACE PROTEIN-2 IS SEROGROUP SPECIFIC AND PREDOMINANTLY OF THE IMMUNOGLOBULIN G3 SUBCLASS
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DOI：
10.1128/iai.63.11.4382-4388.1995
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发表时间：
1995-11-01
影响因子：
3.1
通讯作者：
RILEY, EM
中科院分区：
文献类型：
--
作者：
TAYLOR, RR;SMITH, DB;RILEY, EM
MSP2是恶性疟原虫裂殖子表面蛋白，因此是疟疾疫苗的潜在成分。在这项研究中，我们使用了一组重组MSP2抗原用于酶联免疫吸附试验，以研究来自疟疾免疫人血清的抗体对MSP2的识别作用。这些重组抗原包括A和B血清组的全长蛋白和代表每个血清组的保守、组特异性或重复区域的片段。95%的受测血清样本含有MSP2特异性抗体：81%的受测血清样本对A组有反应，86%对B组有反应。抗体反应几乎完全针对MSP2的二态和多态区域；保守区很少被识别，针对A组和B组的抗体不发生交叉反应。有趣的是，抗体反应主要是亲细胞和补体固定亚类免疫球蛋白G3。
MSP2 is a merozoite surface protein of Plasmodium falciparum and, as such, is a potential component of a malaria vaccine. In this study, we have used a panel of recombinant MSP2 antigens in enzyme-linked immunosorbent assays to investigate the recognition of MSP2 by antibodies from malaria-immune human serum. These recombinant antigens include full-length proteins of serogroups A and B and fragments representing the conserved, group-specific, or repeat regions of each serogroup. Ninety-five percent of the serum samples tested contained MSP2-specific antibodies: 81% of serum samples tested responded to serogroup A, and 86% responded to serogroup B. The antibody response is directed almost exclusively towards dimorphic and polymorphic regions of MSP2; the conserved regions are rarely recognized, and antibodies to serogroups A and B do not cross-react, Interestingly, the antibody response is predominately of the cytophilic and complement-fixing subclass immunoglobulin G3.