Identification of genes associated with perianth senescence in Daffodil (Narcissus pseudonarcissus L. 'Dutch Master')
Identification of genes associated with perianth senescence in Daffodil (Narcissus pseudonarcissus L. 'Dutch Master')
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DOI：
10.1016/s0168-9452(02)00068-7
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发表时间：
2002-07-01
期刊：
影响因子：
5.2
通讯作者：
Reid, MS
中科院分区：
文献类型：
--
作者：
Hunter, DA;Steele, BC;Reid, MS
我们使用了基于pcr的减法程序来分离早期衰老水仙花（Narcissus pseudonarcissus L.）花被中丰度增加的基因。“荷兰大师”)花。通过RNA凝胶印迹分析，我们证实其中18个转录本与衰老有关。其中一些在先前研究的衰老花和叶组织系统中有同源物（半胱氨酸蛋白酶、丝氨酸蛋白酶、核酸内切酶、液泡加工酶）。其中一些与具有运输功能的蛋白质编码基因（生长素外排载体蛋白，低亲和力硝酸盐转运蛋白）同源，另一些与可能在细胞信号传导中起作用的酶（rsh样蛋白）同源。令人惊讶的是，分离的基因要么与先前报道的序列没有同源性，要么是没有已知功能的序列。在离体水仙花的花被中，一些转录本的积累也比附着花的衰老早，这也表明了它们与衰老的强烈关联。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
We used a PCR-based subtractive procedure to isolate genes that increased in abundance in the perianth of incipiently senescent daffodil (Narcissus pseudonarcissus L. 'Dutch Master') flowers. Using RNA gel blot analysis we confirmed that 18 of the transcripts were senescence-associated. A number of these have homologs in previously-studied senescing floral and leaf tissue systems (cysteine proteases, serine proteases, endonuclease, vacuole processing enzyme). Some showed homology to genes encoding proteins with transport functions (auxin efflux carrier protein, low affinity nitrate transporter), others to enzymes that may have a role in cellular signaling (RSH-like protein). A surprising number of the isolated genes either showed no homology with previously-reported sequences or were sequences with no known function. A strong association of some of the transcripts with senescence was also indicated by their enhanced accumulation in the perianth of detached daffodil flowers, which senesce earlier than attached flowers. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.