Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma
Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma
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DOI：
10.3892/or.2013.2774
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发表时间：
2013-12-01
期刊：
影响因子：
4.2
通讯作者：
Wang, Yunshan
中科院分区：
文献类型：
--
作者：
Ma, Wanshan;Shi, Xiaohong;Wang, Yunshan
低氧诱导因子-1(HIF-1)和分化胚胎软骨细胞表达基因1(DEC1)是保护肝癌细胞免受低氧微环境影响的两个关键因子。然而，关于低氧对肝细胞癌中HIF-1和DEC1表达的影响，目前知之甚少。本研究采用逆转录聚合酶链式反应(RT-PCR)和免疫印迹法检测了常氧和低氧条件下(0，2，4，6，24和48h)CoCl2诱导的HIF-1和DEC1的mRNA和蛋白水平。此外，利用HIF-1蛋白抑制剂YC-1分析了DEC1与HIF-1表达的相互作用及其相关机制。结果表明，与正常肝细胞相比，肝癌组织中DEC1的表达在mRNA和蛋白水平均显著上调(P
Hypoxia-inducible factor-1 (HIF-1) and differentiated embryo-chondrocyte expressed gene 1 (DEC1) are two key factors that protect hepatocellular carcinoma (HCC) cells from a hypoxic microenvironment. However, little is known concerning the effects of hypoxia on the expression of HIF-1 and DEC1 in HCC. In the present study, RT-PCR and western blotting were conducted to assay the mRNA and protein levels of HIF-1 and DEC1 under normoxia and hypoxia induced by exposure to CoCl2 for different time periods (0, 2, 4, 6, 24 and 48 h). In addition, the HIF-1 protein inhibitor, YC-1, was used to analyze the interaction between DEC1 and HIF-1 expression and the related mechanism. Results showed that expression of DEC1 in HCC was significantly upregulated at both the mRNA and protein levels, when compared with that in normal liver cells (P