Noradrenaline increases the expression and release of Hsp72 by human neutrophils
Noradrenaline increases the expression and release of Hsp72 by human neutrophils
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DOI：
10.1016/j.bbi.2010.02.003
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发表时间：
2010-05-01
影响因子：
15.1
通讯作者：
Ortega, E.
中科院分区：
文献类型：
--
作者：
Giraldo, E.;Multhoff, G.;Ortega, E.
细胞外72kda热休克蛋白（eHsp72）血药浓度在应激条件下升高，包括剧烈运动。然而，其释放到血液中的信号、来源和分泌途径尚不清楚。本研究的目的是评估去甲肾上腺素（NA）作为应激信号对循环中性粒细胞（作为来源）表达和释放Hsp72的作用，所有这些都是在久坐和健康的年轻（21-26岁）女性在运动诱导应激期间的免疫生理调节背景下进行的。流式细胞术检测Hsp72在离体中性粒细胞表面的表达，ELISA法检测其在离体培养中性粒细胞中的释放量。cmHsp70-FITC孵育表明，中性粒细胞在基础条件下表面表达Hsp72。此外，分离培养的中性粒细胞（37℃，5% CO2）在基础条件下也会释放Hsp72，在没有细胞损伤的情况下，释放量从10 min增加到24 h。NA浓度为10(-9)-10(-5)M，孵育60 min和24 h后，表达Hsp72的中性粒细胞百分比增加了一倍。NA在孵育10分钟后也刺激了Hsp72的释放（约20%）。综上所述：(1)在基础条件下，Hsp72在分离的中性粒细胞表面表达，并被NA增强。(2)在培养的基础条件下，在没有细胞死亡的情况下，分离的中性粒细胞也可以释放Hsp72， NA可以增加这种释放。这些结果可能有助于证实NA可以作为运动应激背景下eHsp72增加的“应激信号”的假设，中性粒细胞作为表达来源的作用，以及在较小程度上，NA激活后Hsp72的释放。(C) 2010爱思唯尔公司版权所有。
The blood concentration of extracellular 72 kDa heat shock protein (eHsp72) increases under conditions of stress, including intense exercise. However, the signal(s), source(s), and secretory pathways in its release into the bloodstream have yet to be clarified. The aim of the present study was to evaluate the role of noradrenaline (NA) as a stress signal on the expression and release of Hsp72 by circulating neutrophils (as a source), all within a context of the immunophysiological regulation during exercise-induced stress in sedentary and healthy young (21-26 years) women. The expression of Hsp72 on the surface of isolated neutrophils was determined by flow cytometry, and its release by cultured isolated neutrophils was determined by ELISA. Incubation with cmHsp70-FITC showed that neutrophils express Hsp72 on their surface under basal conditions. In addition, cultured isolated neutrophils (37 degrees C and 5% CO2) also released Hsp72 under basal conditions, with this release increasing from 10 min to 24 h in the absence of cell damage. NA at 10(-9)-10(-5) M doubled the percentage of neutrophils expressing Hsp72 after 60 min and 24 h incubation. NA also stimulated (by about 20%) the release of Hsp72 after 10 min of incubation. In conclusion: (1) Hsp72 is expressed on the surface of isolated neutrophils under basal conditions, and this expression is augmented by NA. (2) Isolated neutrophils can also release Hsp72 under cultured basal conditions in the absence of cell death, and NA can increase this release. These results may contribute to confirming the hypothesis that NA can act as a "stress signal" for the increased eHsp72 in the context of exercise stress, with a role for neutrophils as a source for the expression and, to a lesser degree, the release of Hsp72 after activation by NA. (C) 2010 Elsevier Inc. All rights reserved.