INHIBITION OF MAMMALIAN RNA-POLYMERASE BY 5,6-DICHLORORIBOFURANOSYLBENZIMIDAZOLE (DRB) AND DRB TRIPHOSPHATE
INHIBITION OF MAMMALIAN RNA-POLYMERASE BY 5,6-DICHLORORIBOFURANOSYLBENZIMIDAZOLE (DRB) AND DRB TRIPHOSPHATE
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DOI：
10.1093/nar/5.9.3325
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发表时间：
1978-01-01
影响因子：
14.9
通讯作者：
HAUSEN, P
中科院分区：
文献类型：
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作者：
DREYER, C;HAUSEN, P
DRB三磷酸抑制分离的RNA聚合酶B的活性，并在较小程度上抑制聚合酶A的活性。这同样适用于在分离的细胞核中的转录。它不起起始抑制剂的作用。在所有情况下，都需要高浓度的DRB三磷酸盐。细胞不会将DRB磷酸化到可测量的程度。抗DRB的hnRNA在药物存在下用ATP和GTP两者起始。这些实验使得细胞中的DR B三磷酸特异性干扰聚合酶B的起始反应的假设不太可能。
DRB triphosphate inhibits activity of isolated RNA polymerase B, and, to a lesser extent, that of polymerase A. The same holds true for transcription in isolated nuclei. It does not act as an initiation inhibitor. In all cases, high concentrations of DRB triphosphate are required. Cells do not phosphorylate DRB to a measurable extent. hn RNA resistant to DRB is initiated with both ATP and GTP in the presence of the drug. These experiments render the hypothesis unlikely that DRB triphosphate in the cell specifically interferes with the initiation reaction of polymerase B.