Genetic dissection of seed appearance quality using recombinant inbred lines in soybean
Genetic dissection of seed appearance quality using recombinant inbred lines in soybean
复制标题
利用大豆重组自交系对种子外观品质进行遗传解析
DOI：
10.1007/s11032-021-01262-9
复制
发表时间：
2021-12-01
影响因子：
3.1
通讯作者：
Sun, Lianjun
中科院分区：
文献类型：
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作者：
Hu, Quan;Zhang, Yanwei;Sun, Lianjun
大豆种子的外观质量对商品性影响很大。本研究的目的是鉴定控制大豆种子外观品质的数量性状基因座（QTL）。利用齐黄34号×冀豆17号共256个重组自交系进行QTL定位。我们创新性地应用了机器视觉系统来量化每一行的种子外观。作为QTL定位的结果，在三个环境中共检测到145个机器视觉参数的QTL。对重叠定位的QTL进行整合，共获得16个QTL热点。在这些热点中，热点-4-1被认为是控制种子大小的主要位点，热点-15被认为影响种子颜色和质地。本研究全面挖掘了大豆品种种子外观品质的QTL，为大豆种子外观品质的表型分析提供了一种有效的方法。这些结果将有助于深入剖析大豆种子外观品质的遗传基础，为大豆的改良提供理论依据。
Soybean seed appearance quality greatly affects the marketability. The objective of this study was to identify the quantitative trait loci (QTLs) that control the appearance quality of soybean seeds. A total of 256 recombinant inbred lines from Qi Huang No.34 × Ji Dou No.17 were utilized for QTL mapping. We innovatively applied a machine vision system to quantify the seed appearance of each line. As a result of QTL mapping, a total of 145 QTLs for the machine vision parameters were detected across three environments. We integrated QTLs mapped overlapped and obtained 16 QTL hotspots in total. Of these hotspots,hotspot-4–1was suggested to be a major locus controlling seed size, andhotspot-15was identified to affect the seed color and texture. The mapping for principal components of the seed appearance also supported it. This study comprehensively dug up the QTLs for seed appearance quality of soybean cultivars while providing an efficient method for phenotyping of seed appearance. These results would contribute to dissecting the genetic bases of seed appearance quality for the improvement of soybean.