Odontoblast-like cell differentiation and dentin formation induced with TGF-β1
Odontoblast-like cell differentiation and dentin formation induced with TGF-β1
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DOI：
10.1016/j.archoralbio.2011.05.002
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发表时间：
2011-11-01
影响因子：
3
通讯作者：
Shi, Junnan
中科院分区：
文献类型：
--
作者：
Li, Yucheng;Lu, Xin;Shi, Junnan
目的：目的：探讨支架材料与转化生长因子-β 1（transforminggrowthfactor-beta 1，TGF-β 1）联合诱导牙髓细胞向成牙本质细胞分化及牙本质形成的可能性。将成团细胞置于与TGF-β 1结合或不与TGF-β 1结合的滤器上，进行体外或体内培养。结果：茜素红染色和碱性磷酸酶（ALP）活性测定显示TGF-β 1可促进牙髓细胞矿化和ALP活性。体外培养7 d后，TGF-β 1组细胞出现分化，表达牙本质涎蛋白（DSP）、骨桥蛋白（OPN）和I型胶原（Col I）。体内移植7 d后，实验组滤器表面形成柱状成牙本质细胞，移植3个月后形成表达DSP的管状牙本质。结论：TGF-β 1联合transfilter可诱导成牙本质细胞分化和牙本质形成。本研究结果提示，在设计诱导牙本质组织工程的支架材料时，应考虑合适的成牙本质细胞祖细胞锚的基质和启动成牙本质细胞分化的诱导信号。(C)2011爱思唯尔有限公司保留所有权利。
Objective: To investigate the inductive potential of scaffold material combing with transforming growth factor-beta 1 (TGF-beta 1), and to induce odontoblast differentiation and dentin formation from dental pulp cells both in vitro and in vivo.Methods: Primarily cultured dental pulp cells were used for MTT, ALP activity assay and Alizarin red staining in the presence of TGF-beta 1. Pelleted cells were put on the filters combining with or not with TGF-beta 1 and cultured in vitro or in vivo. The in vitro and in vivo cell response and tissue formation were analysed with Haematoxylin-Eosin (HE), transmission electron microscopy (TEM) and immunohistochemical staining.Results: TGF-beta 1 increased the mineralization and ALP activity of dental pulp cells as revealed by Alizarin red staining and ALP activity assay. After in vitro culture for 7 days, cells polarized in the TGF-beta 1 group and expressed dentin sialoprotein (DSP), osteopotin (OPN) and type I collagen (Col I). After in vivo transplantation for 7 days, columnar odontoblast formed on the surface of filter in experimental group, and tubular dentin expressing DSP formed after 3 months transplantation.Conclusion: It was concluded that TGF-beta 1 combining with transfilter could induce odontoblast differentiation and dentin formation. Our results implied that suitable substrate for the progenitors of odontoblast to anchor on and inductive signals to initiate the differentiation of odontoblast should be taken into consideration when designing scaffold material for inducing dentin tissue engineering. (C) 2011 Elsevier Ltd. All rights reserved.