Characterization of chicken T cell growth factor
Characterization of chicken T cell growth factor
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鸡 T 细胞生长因子的表征
DOI：
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发表时间：
1983
影响因子：
5.4
通讯作者：
R. Franklin
中科院分区：
文献类型：
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作者：
M. Schnetzler;A. Oommen;J. Nowak;R. Franklin
用植物血凝素或刀豆蛋白A(ConA)刺激鸡脾和外周血淋巴细胞，检测其产生T细胞生长因子(IL-2)的能力。用微量定性测定法，在有丝分裂原刺激的淋巴细胞培养上清液中发现活性峰值。这些培养上清液可使鸡淋巴细胞持续生长数周，并增强鸡胸腺细胞的ConA反应。建立了一种定量的Probit微量分析方法，并在适当的情况下用于进一步的研究。鸡IL-2能与胸腺细胞和活化的T细胞特异结合，其对T母细胞的作用不能被环孢素A阻断。天然IL-2制剂凝胶过滤发现两个生物活性峰的相对分子质量在19500-21500和9000-11500之间。在还原条件下，凝胶电泳法测得一条相对分子质量为13000的多肽。
Chicken spleen and peripheral blood lymphocytes were tested for their ability to produce T cell growth factor (interleukin 2, IL2) upon stimulation with phytohemagglutinin or concanavalin A (Con A). Using a qualitative microassay, peak activity was found in 24‐h supernatants of mitogen‐stimulated lymphocytes. These supernatants permitted continuous growth of chicken lymphocytes for several weeks and increased the Con A response of chicken thymocytes. A quantitative probit microassay was developed and used in the further studies when appropriate. Chicken IL2 binds specifically to thymocytes and activated T cells and its action upon T blast cells cannot be blocked by cyclosporin A. The molecular weights of the two biological active peaks found by gel filtration of the native IL 2 preparation were in the range 19500‐21500 and 9000‐11500. A single polypeptide of molecular weight 13000 was found by gel electrophoresis under reducing conditions.