Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets
Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets
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DOI：
10.1093/jn/137.2.315
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发表时间：
2007-02-01
影响因子：
4.2
通讯作者：
Wu, Guoyao
中科院分区：
文献类型：
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作者：
Frank, Jason W.;Escobar, Jeffery;Wu, Guoyao
本研究探讨了口服补充N-氨甲酰谷氨酸（NCG），内源性N-乙酰谷氨酸（精氨酸合成的激活剂）的类似物增加母猪饲养仔猪生长速度的潜在机制。每头泌乳母猪随机取2头体重相等、性别相同的仔猪，按0（对照组）或50环/kg体重的NCG剂量灌胃，每12 h 1次，连续7 d。仔猪（n = 32，BW = 3 kg）进行了研究，在7天的治疗后，在食物剥夺或进食状态。隔夜食物剥夺仔猪给予NCG或水（对照）在时间0和60分钟。在喂养状态下研究的仔猪管饲母猪的牛奶与其各自的NCG治疗在0和60分钟。在60分钟，仔猪给予洪水剂量的[H-3]苯丙氨酸，并在90分钟杀死，以测量组织蛋白质合成。在7天内，NCG组仔猪增重比对照组高28%（P < 0.001）。与禁食猪相比，进食猪的背最长肌、腓肠肌和十二指肠蛋白质合成率更高（P < 0.001）。与对照组相比，NCG处理组背最长肌（P = 0.050）和腓肠肌（P = 0.068）肌肉的绝对蛋白质合成率分别高出30%和21%。补充NCG的仔猪血浆精氨酸和生长激素浓度也显著高于对照组（P < 0.001）。结果表明，口服NCG补充增加血浆精氨酸和生长激素水平，导致哺乳仔猪的生长速度和肌肉蛋白质合成的增加。
This study investigated the potential mechanisms by which oral supplementation of N-carbamylglutamate (NCG), an analogue of endogenous N-acetylglutamate (an activator of arginine synthesis) increases growth rate in sow-reared piglets. Two piglets of equal body weight (BW) and of the same gender from each lactating sow were allotted to receive oral administration of 0 (control) or 50 ring of NCG/kg BW every 12 h for 7 d. Piglets (n = 32, BW = 3 kg) were studied in the food-deprived or fed state following the 7 d of treatment. Overnight food-deprived piglets were given NCG or water (control) at time 0 and 60 min. Piglets studied in the fed state were gavage-fed sow's milk with their respective NCG treatment at 0 and 60 min. At 60 min, the piglets were administered a flooding dose of [H-3]phenylalanine and killed at 90 min to measure tissue protein synthesis. Piglets treated with NCG gained 28% more weight than control pigs (P < 0.001) over the 7-d period. Fed pigs had greater rates of protein synthesis in longissimus dorsi and gastrocnemius muscles and duodenum compared with food-deprived pigs (P < 0.001). Absolute protein synthesis rates in longissimus dorsi (P = 0.050) and gastrocnemius (P = 0.068) muscles were 30 and 21% greater, respectively, in NCG-treated compared with control pigs. Piglets supplemented with NCG also had greater plasma concentrations of arginine and somatotropin than control pigs (P < 0.001). The results suggest that oral NCG supplementation increases plasma arginine and somatotropin levels, leading to an increase in growth rate and muscle protein synthesis in nursing piglets.