Potential cellular targets and antibacterial efficacy of atmospheric pressure non-thermal plasma
Potential cellular targets and antibacterial efficacy of atmospheric pressure non-thermal plasma
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DOI：
10.1016/j.ijantimicag.2013.08.022
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发表时间：
2014-02-01
影响因子：
10.8
通讯作者：
Gilmore, Brendan F.
中科院分区：
文献类型：
--
作者：
Alkawareek, Mahmoud Y.;Gorman, Sean P.;Gilmore, Brendan F.
常压低温等离子体(APNTP)作为一种新型的抗菌方法正受到越来越多的关注。虽然这种方法显示了良好的抗菌活性，但其确切的作用机制尚不清楚。从机理上阐明抗菌活性将促进这一方法的开发和合理优化，以实现潜在的医疗应用。在这项研究中，评估了内部制造的APNTP喷射器的抗菌效果，同时调查了APNTP与主要细胞成分之间的相互作用，以确定参与血浆介导的细菌破坏机制的潜在细胞靶点。所研究的等离子喷射器对一组选定的临床重要细菌具有良好的快速抗菌活性，包括蜡状芽孢杆菌、耐甲氧西林金黄色葡萄球菌(MRSA)、大肠杆菌和铜绿假单胞菌，所有这些细菌在等离子体暴露后2分钟内均被完全灭活。观察到不同程度的血浆介导的损伤对所有被研究的细胞成分，包括DNA，一种模型蛋白质酶，以及脂膜完整性和通透性。APNTP的抗菌效果似乎涉及一种多靶点机制，这可能会降低微生物对这种有希望的抗菌方法产生耐药性的可能性。然而，细胞膜损伤和由此引起的通透性扰动被发现是这一机制中最有可能的速率决定步骤。皇冠版权所有(C)2013，由爱思唯尔公司代表国际化疗学会出版。版权所有。
Atmospheric pressure non-thermal plasma (APNTP) has been gaining increasing interest as a new alternative antibacterial approach. Although this approach has demonstrated promising antibacterial activity, its exact mechanism of action remains unclear. Mechanistic elucidation of the antimicrobial activity will facilitate development and rational optimisation of this approach for potential medical applications. In this study, the antibacterial efficacy of an in-house-built APNTP jet was evaluated alongside an investigation of the interactions between APNTP and major cellular components in order to identify the potential cellular targets involved in plasma-mediated bacterial destruction mechanisms. The investigated plasma jet exhibited excellent, rapid antibacterial activity against a selected panel of clinically significant bacterial species including Bacillus cereus, meticillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Pseudomonas aeruginosa, all of which were completely inactivated within 2 min of plasma exposure. Plasma-mediated damaging effects were observed, to varying degrees, on all of the investigated cellular components including DNA, a model protein enzyme, and lipid membrane integrity and permeability. The antibacterial efficacy of APNTP appears to involve a multiple-target mechanism, which potentially reduces the likelihood of emergence of microbial resistance towards this promising antimicrobial approach. However, cellular membrane damage and resulting permeability perturbation was found to be the most likely rate-determining step in this mechanism. Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of International Society of Chemotherapy. All rights reserved.