Blue light and phytochrome-mediated stomatal opening in the npq1 and phot1 phot2 mutants of Arabidopsis
Blue light and phytochrome-mediated stomatal opening in the npq1 and phot1 phot2 mutants of Arabidopsis
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DOI：
10.1104/pp.103.029587
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发表时间：
2003-12-01
期刊：
影响因子：
7.4
通讯作者：
Zeiger, E
中科院分区：
文献类型：
--
作者：
Talbott, LD;Shmayevich, IJ;Zeiger, E
最近的研究表明，蓝光特定的气孔开放被绿色光逆转，远红光可以用来探测光敏色素依赖的气孔运动。在这里，蓝绿色可逆性和远红光探测气孔响应的npq 1突变体和phot 1 phot 2双突变体的拟南芥。在50 mumol m（-2）s（-1）下生长的植物中，红光（光合作用）介导的野生型（WT）和两种突变体的孤立气孔在100 mumol m（-2）s（-1）下饱和开放。较高的能量密度率造成气孔关闭，最有可能是由于光抑制。在100 mumol m（-2）s（-1）红色背景中加入蓝光（10 mumol m（-2）s（-1）），观察到野生型气孔具有蓝光特异性开放，但在npq 1或phot 1 phot 2双突变气孔中没有发现。在50 mumol m（-2）s（-1）红光和10 mumol m（-2）s（-1）蓝光下，野生型和npql突变体的气孔都能打开，而phot 1 phot 2双突变体的气孔则不能打开。在npql中，蓝光刺激的开放被远红光而不是绿色光逆转，表明npql具有光敏色素介导的反应，缺乏蓝光特异性反应。phot 1 phot 2双突变体的气孔对20 - 50 mumol m（-2）s（-1）蓝光有反应。这种开放是绿色光可逆和远红光不敏感，表明气孔的phot 1 phot 2双突变体有一个可检测的蓝光特异性反应。
Recent studies have shown that blue light-specific stomatal opening is reversed by green light and that far-red light can be used to probe phytochrome-dependent stomatal movements. Here, blue-green reversibility and far-red light were used to probe the stomatal responses of the npq1 mutant and the phot1 phot2 double mutant of Arabidopsis. In plants grown at 50 mumol m(-2) s(-1), red light (photosynthetic)-mediated opening in isolated stomata from wild type (WT) and both mutants saturated at 100 mumol m(-2) s(-1). Higher fluence rates caused stomatal closing, most likely due to photo-inhibition. Blue light-specific opening, probed by adding blue light (10 mumol m(-2) s(-1)) to a 100 mumol m(-2) s(-1) red background, was found in WT, but not in npq1 or phot1 phot2 double mutant stomata. Under 50 mumol m(-2) s(-1) red light, 10 mumol m(-2) s(-1) blue light opened stomata in both WT and npql mutant stomata but not in the phot1 phot2 double mutant. In npql, blue light-stimulated opening was reversed by far-red but not green light, indicating that npql has a phytochrome-mediated response and lacks a blue light-specific response. Stomata of the phot1 phot2 double mutant opened in response to 20 to 50 mumol m(-2) s(-1) blue light. This opening was green light reversible and far-red light insensitive, indicating that stomata of the phot1 phot2 double mutant have a detectable blue light-specific response.