CHARACTERIZATION OF THE STAPHYLOCOCCUS-AUREUS CHROMOSOMAL GENE PCRA, IDENTIFIED BY MUTATIONS AFFECTING PLASMID-PT181 REPLICATION
CHARACTERIZATION OF THE STAPHYLOCOCCUS-AUREUS CHROMOSOMAL GENE PCRA, IDENTIFIED BY MUTATIONS AFFECTING PLASMID-PT181 REPLICATION
复制标题
DOI：
10.1007/bf00280216
复制
发表时间：
1993-10-01
期刊：
影响因子：
--
通讯作者：
IORDANESCU, S
中科院分区：
文献类型：
--
作者：
IORDANESCU, S
金黄色葡萄球菌染色体基因 pcrA 已被克隆并测序，该基因通过影响质粒 pT181 复制的 pcrA3 等突变来鉴定。 pcrA 基因编码的蛋白质与两种大肠杆菌解旋酶具有显着相似性（40% 同一性）：uvrD 基因编码的解旋酶 II 和 Rep 解旋酶。发现 pcrA3 突变是 C 到 T 的转变，导致蛋白质的氨基酸残基 61 处苏氨酸被异亮氨酸取代。 pcrA 基因似乎属于一个操纵子，该操纵子包含至少一个其他基因，暂时命名为 pcrB，位于 pcrA 的上游。 PcrA 蛋白被证明对于细胞活力至关重要，过量生产会对宿主和质粒复制产生有害影响。
The Staphylococcus aureus chromosomal gene pcrA, identified by mutations, such as pcrA3, that affect plasmid pT181 replication, has been cloned and sequenced. The pcrA gene encodes a protein with significant similarity (40% identity) to two Escherichia coli helicases: the helicase II encoded by the uvrD gene and the Rep helicase. The pcrA3 mutation was found to be a C to T transition leading to a threonine to isoleucine substitution at amino acid residue 61 of the protein. The pcrA gene seems to belong to an operon containing at least one other gene, tentatively named pcrB, upstream from pcrA. The PcrA protein was shown to be essential for cell viability and overproduction has deleterious effects on the host and plasmid replication.