THE EFFECT OF EXTRACELLULAR CALCIUM ELEVATION ON MORPHOLOGY AND FUNCTION OF ISOLATED RAT OSTEOCLASTS
THE EFFECT OF EXTRACELLULAR CALCIUM ELEVATION ON MORPHOLOGY AND FUNCTION OF ISOLATED RAT OSTEOCLASTS
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DOI：
10.1007/bf01114813
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发表时间：
1989-12-01
影响因子：
4
通讯作者：
ZAIDI, M
中科院分区：
文献类型：
--
作者：
DATTA, HK;MACINTYRE, I;ZAIDI, M
破骨细胞是一种大型多核细胞，具有独特的骨吸收能力。这些细胞在再吸收过程中局部暴露于高水平的离子化钙。因此，我们研究了细胞外钙离子升高对新鲜分解的大鼠破骨细胞的形态和功能的影响。通过延时录像、数字化和计算机图像分析对细胞大小和运动性进行定量。为了评估孤立的破骨细胞的吸收能力，我们测量了总面积的吸收失活皮质骨的扫描电子显微镜和计算机为基础的形态测量。结果表明，细胞外钙离子浓度的升高导致细胞大小急剧减小，伴随着酶释放的显著减少和骨吸收的消除。我们认为钙离子可能在骨细胞骨吸收的局部调节中起重要作用。
Osteoclasts are large multinucleate cells unique in their capacity to resorb bone. These cells are exposed locally to high levels of ionised calcium during the process of resorption. We have therefore examined the effect of elevated extracellular calcium on the morphology and function of freshly disaggregated rat osteoclasts. Cell size and motility were quantitated by time-lapse video recording together with digisation and computer-centred image analysis. In order to assess the resorptive capacity of isolated osteoclasts, we measured the total area of resorption of devitalised cortical bone by means of scanning electron microscopy and computer-based morphometry. The results show that elevation of the extracellular calcium concentration causes a dramatic reduction of cell size, accompanied by a marked diminution of enzyme release and abolition of bone resorption. We propose that ionised calcium might play an important role in the local regulation of osteoclastic bone resorption.