A rearrangement resulting in small tandem repeats in the F3′5′H gene of white flower genotypes is associated with the soybean W1 locus
A rearrangement resulting in small tandem repeats in the F3′5′H gene of white flower genotypes is associated with the soybean W1 locus
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DOI：
10.2135/cropsci2006.12.0838tpg
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发表时间：
2007-07-01
期刊：
影响因子：
2.3
通讯作者：
Vodkin, Lila O.
中科院分区：
文献类型：
--
作者：
Zabala, Gracia;Vodkin, Lila O.
大豆[Glycine max（L.）梅尔。]花色素由六个基因座W1、W2、W3、W 4、Wm和Wp控制，其中只有三个基因座的功能是已知的。这三个基因座上的隐性等位基因都不能完全消除花色素。另一方面，W1位点是花色素沉着所必需的。基于遗传和生物化学数据，三羟基化反应形成飞燕草素-3-葡萄糖苷被假定为W7基因座的可能功能。本研究克隆了紫花大豆品系L79-908 W的类黄酮3 '5'-羟化酶（F3 '5'H）基因和白色花大豆品系威廉姆斯的隐性等位基因。该突变是一种重排，导致外显子3中插入一个小的（65 bp）串联重复序列，从而过早地截断翻译产物。在所有的白色花大豆品系和紫色和白色花大豆品系杂交产生的分离群体中，这种插入的存在提供了令人信服的证据，即分离的F3 '5'H基因可能由大豆的W1基因座编码。GmF 3 ′ 5 ′ H是一个单拷贝基因，在所有检测的组织中表达水平非常低，包括花和种皮，但足以解释这些组织中飞燕草苷型花色素苷和/或原花色素苷的贡献。
Soybean [Glycine max (L.) Merr.] flower pigmentation is controlled by six loci, W1, W2, W3, W4, Wm, and Wp, of which the functions of only three are known. None of the recessive alleles at those three loci completely abolishes flower pigmentation. On the other hand, the W1 locus is required for flower pigmentation. Based on genetic and biochemical data, the trihydroxylation reaction to form delphinidin-3-glucoside has been postulated as a likely function of the W7 locus. We report the cloning and sequencing of a flavonoid 3'5'-hydroxylase (F3'5'H) gene from the purple flower soybean line L79-908 W) and the recessive allele from the white flower isoline 'Williams' 41). The mutation is a rearrangement leading to a small (65 bp) insertion of tandem repeats in exon 3 that truncates the translation product prematurely. The presence of this insertion in all-white flower soybean lines examined and in those of a segregating population resulting from a cross between purple and white flower lines presents compelling evidence that the F3'5'H gene isolated is likely encoded by the W1 locus of Glycine max. GmF3'5'H is a single-copy gene, expressed at very low levels in all tissues examined, including flower and seed coats, but sufficient to account for the contribution of the delphinidin-bosed anthocyanins and/or proanthocyanins in these tissues.