Protein uptake by bacteria: An endocytosis-like process in the planctomycete Gemmata obscuriglobus.
Protein uptake by bacteria: An endocytosis-like process in the planctomycete Gemmata obscuriglobus.
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DOI：
10.4161/cib.3.6.13061
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发表时间：
2010-11-01
影响因子：
--
通讯作者：
Sagulenko, Evgeny
中科院分区：
文献类型：
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作者：
Fuerst, John A;Sagulenko, Evgeny
内吞作用是真核生物中膜运输的基本过程，但在细菌或古细菌中还没有发现。内吞作用的起源是理解第一批真核生物及其内膜系统进化的核心。在最近的一项研究中，我们已经确定了一种细菌，Gemmata obscuriglobus，一个独特的浮游菌门的肽聚糖少芽细菌的成员，发生在细胞中的蛋白质摄取的内吞样过程。该门的成员特征性地具有被内膜分隔成室的细胞，在G.这些区室包括一个双层膜包围其核DNA的区室，以及细胞质的外部无核糖体区。蛋白质可以被细胞从外部环境内化并收集到这个无核糖体的隔室中，并且这个过程是能量依赖的，并且似乎是受体介导的。如在真核生物内吞作用中，内化的蛋白质与囊泡相关联，并且可以进行蛋白水解降解。在细菌中发现这一过程对我们理解真核生物中内吞作用的起源具有重要意义。
Endocytosis is a fundamental process of membrane-trafficking in eukaryotes, but has not been known to occur in bacteria or archaea. The origin of endocytosis is central to the understanding of evolution of the first eukaryotes and their endomembrane systems. In a recent study we have established that an endocytosis-like process for uptake of proteins into cells occurs in a bacterium, Gemmata obscuriglobus, a member of the distinctive phylum Planctomycetes of peptidoglycan-less budding bacteria. Members of this phylum characteristically possess cells divided into compartments separated by internal membranes and in the case of G. obscuriglobus these compartments include one where a double membrane envelope surrounds its nucleoid DNA, as well as an outer ribosome- free region of cytoplasm. Proteins can be internalized by cells from the external milieu and collected into this ribosome-free compartment, and this process is energy-dependent and appears to be receptor-mediated. As in eukaryote endocytosis, internalized proteins are associated with vesicles, and can be subjected to proteolytic degradation. The discovery of this process in a bacterium has significant implications for our understanding of the origins of endocytosis in eukaryotes.