Effects of the interaction of single-walled carbon nanotubes with 4-nonylphenol on their in vitro toxicity
Effects of the interaction of single-walled carbon nanotubes with 4-nonylphenol on their in vitro toxicity
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DOI：
10.1016/j.jhazmat.2014.04.055
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发表时间：
2014-06-30
影响因子：
13.6
通讯作者：
Valcarcel, M.
中科院分区：
文献类型：
--
作者：
Caballero-Diaz, E.;Guzman-Ruiz, R.;Valcarcel, M.
本研究的目的是评估聚乙二醇涂层的单壁碳纳米管（SWCNTs-PEG）和已知的环境污染物4-壬基苯酚（NP）共存所产生的毒理学风险。为此，进行体外毒性测定，包括将3 T3-L1细胞（小鼠胚胎成纤维细胞）暴露于单独的SWCNTs-PEG或与NP组合24或48小时。在存在（10%）和不存在血清的情况下进行实验处理，以评估其对SWCNTs-PEG的毒性的影响。尽管结果没有提供SWCNTs-PEG和NP之间协同毒性的明确证据，但使用混合物（SWCNTs-PEG + NP）的一些特定处理导致与单独处理相关的意外的组合毒性。只有在这些情况下，SWCNTs-PEG和NP之间的相互作用才能对所产生的毒性产生协同效应。添加10%血清可能通过空间排斥增加了SWCNTs-PEG在培养基中的稳定性，从而降低了纳米颗粒的毒性。总的来说，血清对细胞具有针对所有处理的“保护作用”：SWCNTs-PEG、NP或它们的混合物（SWCNTs-PEG +NP）。拉曼光谱允许阐明SWCNTs-PEG的细胞内分布。(C)2014爱思唯尔有限公司版权所有。
The aim of this study was to assess the toxicological risks arising from the coexistence of polyethylene glycol coated single-walled carbon nanotubes (SWCNTs-PEG) and a known environmental contaminant: 4-nonylphenol (NP). To this end, in vitro toxicity assays involving the exposure of 3T3-L1 cells (mouse embryonic fibroblasts) to SWCNTs-PEG alone or in combination with NP for 24 or 48 h were performed. Experimental treatments were conducted in both presence (10%) and absence of serum in order to evaluate its influence on the toxicity of SWCNTs-PEG. Although the results provided no unambiguous evidences of synergistic toxicity between SWCNTs-PEG and NP, some specific treatments with mixtures (SWCNTs-PEG + NP) resulted in an unexpected combined toxicity in relation to the individual treatments. Only in those cases the interaction between SWCNTs-PEG and NP could have a synergistic effect on the resulting toxicity. The addition of 10% serum increased the stability of SWCNTs-PEG in the culture medium possibly by steric repulsions and reduced the toxicity of nanoparticles as a result. Overall, the serum had a "protective effect" on cells against all treatments: SWCNTs-PEG, NP or their mixtures (SWCNTs-PEG +NP). Raman spectroscopy allowed the intracellular distribution of SWCNTs-PEG to be elucidated. (C) 2014 Elsevier B.V. All rights reserved.