Analysis of type 1 fimbriae expression in verotoxigenic Escherichia coli:: a comparison between serotypes O157 and O26
Analysis of type 1 fimbriae expression in verotoxigenic Escherichia coli:: a comparison between serotypes O157 and O26
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DOI：
10.1099/00221287-147-1-145
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发表时间：
2001-01-01
期刊：
影响因子：
2.8
通讯作者：
Gally, DL
中科院分区：
文献类型：
--
作者：
Roe, AJ;Currie, C;Gally, DL
先前的研究表明，产维罗毒素的大肠杆菌（VTEC）O157菌株似乎不能表达1型菌毛，尽管其他血清型如O26和O118可以表达。本研究探讨了这种差异的分子基础。该研究证实，在63株VTEC O157菌株中，fimA（fim开关）的调控区域内存在16 bp缺失，但在检测的其他VTEC血清型中不存在。在VTEC O157中，积雪开关仅在相位关闭方向上可检测到。但是在VTEC 026中，在取向上的相位中的开关的检测与甘露糖敏感的酵母凝集的程度相关。修复VTEC O157 fim开关区域中的16 bp缺失恢复了fimA在容许背景中的相位可变表达。非O157 VTEC，特别是O26和O118，可以在牛的致病性，1型菌毛在这和殖民化的作用进行了讨论。
Previous research has shown that verotoxin-producing Escherichia coli (VTEC) O157 strains appear unable to express type 1 fimbriae although other serotypes such as O26 and O118 can. This study has investigated the molecular basis of this difference. The study confirmed the presence of a 16 bp deletion within the regulatory region of fimA (fim switch) in 63 VTEC O157 strains but not in other VTEC serotypes tested. The firn switch was shown to be detectable only in the phase off orientation in VTEC O157. but detection of the switch in the phase on orientation correlated with the degree of mannose-sensitive yeast agglutination in VTEC 026. Repair of the 16 bp deletion in the VTEC O157 fim switch region restored phase-variable expression of fimA in a permissive background. Non-O157 VTEC, especially O26 and O118, can be pathogenic in cattle; the role of type 1 fimbriae in this and colonization is discussed.