Separation parameters via virtual migration distances in high-performance liquid chromatography, capillary zone electrophoresis and electrokinetic chromatography.
Separation parameters via virtual migration distances in high-performance liquid chromatography, capillary zone electrophoresis and electrokinetic chromatography.
复制标题
通过高效液相色谱、毛细管区带电泳和电动色谱中的虚拟迁移距离确定分离参数。
DOI：
10.1016/0021-9673(96)00306-8
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Horváth,C
中科院分区：
文献类型：
--
作者：
Rathore,AS;Horváth,C
在各种不同的分离迁移过程中，高效液相色谱(HPLC)和毛细管区带电泳法(CZE)已成为分离复杂生物物质的两种主要高效分析技术。在具有电内渗流的高效液相色谱和毛细管电泳法中，差异迁移过程可分为分离组分和非分离组分，分离组分涉及与固定相的选择性相互作用或电泳迁移速度的差异，非分离组分代表对流迁移，对分离没有直接贡献。虚拟迁移距离的引入导致了适用于上述两种技术的两个组分的相加关系，并且有助于识别潜在的相似性以及表达和比较各种分离参数。对关键迁移参数的研究导致了电内渗流CZE的各种模式的表征和分类。处理扩展到毛细管电色谱和胶束电动层析的分析，这两个混合过程借鉴了高效液相色谱和毛细管电泳法的特点。虚拟迁移距离的使用也导致了对这些分离系统的特征参数的一致和统一的描述。
Among the various differential migration processes of separation, high-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE) have emerged as the two major high-performance analytical techniques for separation of complex biological substances. In both HPLC and CZE with electroendoosmotic flow, the differential migration process can be divided into a separative component, which involves selective interactions with the stationary phase or differences in the electrophoretic migration velocities, and a non-separative component representing migration by convection that does not contribute directly to separation. The introduction of virtual migration distances leads to an additivity relationship for the two components that is applicable to both of the above techniques and facilitates the recognition of the underlying similarities as well as the expression and comparison of the various separation parameters. Examination of the key migration parameters led to the characterization and the classification of the various modes of CZE with electroendoosmotic flow. The treatment was extended to the analysis of capillary electrochromatography and micellar electrokinetic chromatography; two hybrid processes which exhibit features borrowed from HPLC and CZE. The use of virtual migration distances also led to a consistent and unified description of the characteristic parameters of these separation systems.