Reduced endothelial nitric oxide synthase expression and production in human atherosclerosis
Reduced endothelial nitric oxide synthase expression and production in human atherosclerosis
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DOI：
10.1161/01.cir.97.25.2494
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发表时间：
1998-06-30
期刊：
影响因子：
37.8
通讯作者：
Lüscher, TF
中科院分区：
文献类型：
--
作者：
Oemar, BS;Tschudi, MR;Lüscher, TF
背景-NO调节血管张力和结构、血小板和单核细胞，NO由内皮型一氧化氮合酶(ENOS)合成。方法和结果--用一种卟啉微传感器，在手术中获得的动脉粥样硬化的人的颈动脉和正常的乳动脉中，测量了NO的释放。免疫组织化学方法检测eNOS蛋白表达。在正常动脉，钙离子载体A23187(10mU/L)刺激后NO的初始释放速率为0.42+/-0.05(mU/L)/S(n=10)。而动脉粥样硬化节段NO初始释放速率显著降低，降至0.08+/-0.04(MU/L)/S(n=10，P
Background-NO regulates vascular tone and structure, platelets, and monocytes, NO is synthesized by endothelial NO synthase (eNOS). Endothelial dysfunction occurs in atherosclerosis.Methods and Results-With a porphyrinic microsensor, NO release was measured in atherosclerotic human carotid arteries and normal mammary arteries obtained during surgery. eNOS protein expression was analyzed by immunohistochemistry. In normal arteries, the initial rate of NO release after stimulation with calcium ionophore A23187 (10 mu mol/L) was 0.42 +/- 0.05 (mu mol/L)/s (n=10). In contrast, the initial rate of NO release was markedly reduced in atherosclerotic segments, to 0.08 +/- 0.04 (mu mol/L)/s (n = 10, P