DANCE, a novel secreted RGD protein expressed in developing, atherosclerotic, and balloon-injured arteries
DANCE, a novel secreted RGD protein expressed in developing, atherosclerotic, and balloon-injured arteries
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DOI：
10.1074/jbc.274.32.22476
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发表时间：
1999-08-06
影响因子：
4.8
通讯作者：
Honjo, T
中科院分区：
文献类型：
--
作者：
Nakamura, T;Ruiz-Lozano, P;Honjo, T
我们已经鉴定和鉴定了小鼠、大鼠和人类的cDNA，它们编码一种新的由448个氨基酸组成的分泌性蛋白，命名为DIANE(发育动脉和神经脊表皮生长因子(EGF)样蛋白)。Dance包含六个钙结合的EGF样结构域，其中一个包含RGD基序。对重组舞蹈蛋白的过表达研究表明，舞蹈是一种66 kDa的分泌型蛋白质。Dance和最近描述的蛋白S1-5组成了一个新的EGF样蛋白家族。人类舞蹈基因定位在染色体14q32.1上。在成人组织中，Dance基因主要在心脏、卵巢和结肠组织中表达。原位杂交表达谱分析显示，发育中的动脉中有明显的DIZE表达。Dance也在神经脊细胞衍生物、心内膜垫组织和其他几种间质组织中表达。在成年血管中，DISE的表达在很大程度上被减弱，但在球囊损伤的血管和动脉粥样硬化病变中重新诱导，特别是在损伤后期失去增殖能力的内膜血管平滑肌细胞和内皮细胞中。Dance蛋白通过整合素与DISE的RGD基序相互作用而促进内皮细胞的黏附。因此，Dance是一种新的整合素受体的血管配体，可能在血管发育和重塑中发挥作用。
We have identified and characterized mouse, rat, and human cDNAs that encode a novel secreted protein of 448 amino acids named DANCE (developmental arteries and neural crest epidermal growth factor (EGF)-like). DANCE contains six calcium-binding EGF-like domains, one of which includes an RGD motif. Overexpression studies of recombinant DANCE protein document that DANCE is a secreted 66-kDa protein. DANCE and recently described protein S1-5 comprise a new EGF-like protein family. The human DANCE gene was mapped at chromosome 14q32.1. DANCE mRNA is mainly expressed in heart, ovary, and colon in adult human tissues. Expression profile analysis by in situ hybridization revealed prominent DANCE expression in developing arteries. DANCE is also expressed in neural crest cell derivatives, endocardial cushion tissue, and several other mesenchymal tissues. In adult vessels, DANCE expression is largely diminished but is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells that lose their ability to proliferate in late stage of injury. DANCE protein was shown to promote adhesion of endothelial cells through interaction of integrins and the RGD motif of DANCE. DANCE is thus a novel vascular ligand for integrin receptors and may play a role in vascular development and remodeling.