Control of maintenance and anteroposterior skeletal differentiation of the anterior mesenchyme of the chick wing bud by its posterior margin (the ZPA).
Control of maintenance and anteroposterior skeletal differentiation of the anterior mesenchyme of the chick wing bud by its posterior margin (the ZPA).
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通过后缘（ZPA）控制鸡翅芽前间质的维持和前后骨骼分化。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
M. Gumpel
中科院分区：
文献类型：
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作者：
J. Hinchliffe;M. Gumpel
如果小鸡翅芽的后半部分（第17-22期）被切除，通常形成肱骨（部分）、桡骨和第2趾的前半部分仅形成单个骨骼元件，要么是肱骨，要么是肱骨与缩小的半径融合。从术后18 h开始，持续至48 h，前半部分的前间质和远端间质坏死，AER消退。相比之下，如果鸡翅芽的前半部分（阶段17-22）被切除，后半部分的发育与正常芽一样，并形成肱骨（部分），尺骨和第3，4和5趾。这样的后半部发展没有更多的间充质坏死比正常的对侧翅芽和AER保持健康。此外，如果切除后部分的方式是在适当的位置留下一部分的极化活动区（ZPA），一个正常的翅膀与完整的骨架形成。因此，为了生存和分化，翅芽的前部需要由含有ZPA的后部提供的因子。这些结果支持ZPA在控制正常翅芽前后分化中起作用的观点。
If the posterior half of the chick wing bud (stages 17-22) is excised, the anterior half which normally forms humerus (part), radius and digit 2, forms only a single skeletal element, either humerus or humerus fused with reduced radius. Beginning at 18 h after operation, and continuing to 48 h the anterior and distal mesenchyme in such anterior halves becomes necrotic and the AER regresses. By contrast, if the anterior half of the chick wing bud (stages 17-22) is excised, the posterior half develops as in the normal bud, and forms humerus (part), ulna and digits 3, 4 and 5. Such posterior halves develop no more mesenchymal necrosis than the normal contralateral wing buds and the AER remains healthy. Further, if the excision of the posterior part is made in such a way as to leave in place a part of the zone of polarising activity (ZPA), a normal wing with complete skeleton is formed. Thus in order to survive and differentiate, the anterior part of the wing bud needs a factor supplied by the posterior part containing the ZPA. These results support the view that the ZPA plays a role in controlling the anteroposterior differentiation of the normal wing bud.