Effect of the brachypod mutation on cell adhesion and chondrogenesis in aggregates of mouse limb mesenchyme
Effect of the brachypod mutation on cell adhesion and chondrogenesis in aggregates of mouse limb mesenchyme
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短足突变对小鼠肢体间充质聚集体细胞粘附和软骨形成的影响
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
W. A. Elmer
中科院分区：
文献类型：
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作者：
J. Duke;W. A. Elmer
在旋转培养物中，研究了十二天的正常和臂杆肢的肢体另一方面，骨料及其细胞密度相同。在24小时的培养物中，培养物的组织型24小时。讨论了与软骨生成过程的接触密度和细胞密度。
Twelve-day normal and brachypod mouse limb mesenchyme was studied in rotation culture. Over a 3½ h period the rate of decline of single cells was significantly greater in mutant than in normal cultures, probably because the brachypod cells were more adhesive. However, the final size of the aggregates and their cell densities were the same by 24 h of incubation. On the other hand their pattern of chondrogenesis was different. Normal aggregates contained condensations with typical histotypic cartilage by 24 h of incubation, and were entirely chondrifled by 4 days in culture. The condensations in brachypod aggregates were fewer, smaller, and delayed in their chondrogenesis. Never more than 50% of the brachypod aggregate exhibited chondrogenesis. The importance of cell contact and cell density to the chondrogenic process are discussed.