Oxidative stress markers in tears of patients with Graves' orbitopathy and their correlation with clinical activity score
Oxidative stress markers in tears of patients with Graves' orbitopathy and their correlation with clinical activity score
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DOI：
10.1186/s12886-018-0969-x
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发表时间：
2018-11-21
影响因子：
2
通讯作者：
Yoon, Kyung Chul
中科院分区：
文献类型：
--
作者：
Choi, Won;Li, Ying;Yoon, Kyung Chul
背景资料：探讨Graves' orbitopathy（GO）患者泪液中氧化应激标志物8-羟基-2-脱氧喹核苷（8-OHdG）和丙二醛（MDA）的浓度及其与临床活动评分（CAS）的相关性。方法：我们招募了27名非活动期GO患者、35名活动期GO患者和25名健康对照者。采用酶联免疫吸附法测定泪液中8-OHdG和MDA的含量。根据GO患者病情活动程度分析CAS与泪液8-OHdG、MDA浓度的相关性。对照组8-OHdG和MDA水平分别为56.30+/-16.81 ng/mL和5.39 +/- 1.31 pmol/mg，非活动期GO患者分别为123.46 +/- 22.67 ng/mL和13.59 +/- 3.93 pmol/mg，活动期GO患者分别为215.14 +/- 35.61 ng/mL和22.52 +/- 4.63 pmol/mg。与对照组相比，活动期和非活动期GO患者的8-OHdG和MDA水平均升高（均P <0.05）。
Background: To investigate the concentrations of oxidative stress markers, 8-hydroxy-2-deoxyquanosine (8-OHdG) and malondialdehyde (MDA), in tears and their correlation with the clinical activity score (CAS) in patients with Graves' orbitopathy (GO) according to disease activity.Methods: We recruited 27 participants with inactive stage GO, 35 participants with active stage GO, and 25 healthy controls without GO. The tear concentrations of 8-OHdG and MDA were determined by enzyme-linked immunosorbent assay. The correlation between CAS and the concentrations of tear 8-OHdG and MDA were analyzed according to the disease activity in the GO patients.Results: The levels of 8-OHdG and MDA were 56.30+/-16.81 ng/mL and 5.39 +/- 1.31 pmol/mg, respectively, in the control subjects, and 123.46 +/- 22.67 ng/mL and 13.59 +/- 3.93 pmol/mg, respectively, in patients with inactive stage GO, and 215.14 +/- 35.61 ng/mL and 22.52 +/- 4.63 pmol/mg, in patients with active stage GO. The mean concentrations of 8-OHdG and MDA were higher in patients with inactive and active stage GO compared with the control group (all P