Three Characteristics Associated with Enterotoxigenic Escherichia coli Isolated from Man
Three Characteristics Associated with Enterotoxigenic Escherichia coli Isolated from Man
复制标题
与从人体分离的产肠毒素大肠杆菌相关的三个特征
作者：
D. Evans;D. Evans
在孟加拉国达卡，从许多医院急性霍乱样腹泻病例中分离出产生强效肠毒素的大肠杆菌。利用产肠毒素（tox+）和非产肠毒素（tox -）大肠杆菌分离株来研究可能的区分产肠毒素（tox+）的方法。从那些（毒素−）正常菌群中分离出大肠杆菌。大多数（81%）的tox+E。所研究的大肠杆菌对蔗糖发酵呈阴性，85%的大肠杆菌在pH为8.5的蛋白胨培养基中生长迟缓，92%的大肠杆菌在培养上清液中释放出大量硫酸铵沉淀物质；66.6%的人表现出这三种性质。对于tox组，分别为50%、31%和34%；只有9.3%的人同时具备这三种特性。这些结果表明，除了抗原组成和肠毒素产生外，还可以利用表型特征来鉴定产肠毒素大肠杆菌。
In Dacca, Bangladesh, potent enterotoxin-producing Escherichia coli were isolated from many hospital cases of acute cholera-like diarrhea. Enterotoxigenic (tox+) and non-enterotoxigenic (tox−) isolates of E. coli were used to investigate possible means of differentiating tox+E. coli from those (tox−) of the normal flora. The majority (81%) of the tox+E. coli studied were found to be negative for sucrose fermentation, 85% exhibited retarded growth in a peptone medium at pH 8.5, and 92% released large amounts of ammonium sulfate precipitable materials into culture supernatant fluids; 66.6% exhibited all three of these properties. For the tox− group the respective values were found to be 50%, 31%, and 34%; only 9.3% exhibited all three properties. These results indicate that it may be possible to use phenotypic characteristics other than antigenic composition and enterotoxin production for the identification of enterotoxigenic E. coli.