SPECTRA, MEMBRANE-BINDING, AND POTENTIOMETRIC RESPONSES OF NEW CHARGE SHIFT PROBES
SPECTRA, MEMBRANE-BINDING, AND POTENTIOMETRIC RESPONSES OF NEW CHARGE SHIFT PROBES
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DOI：
10.1021/bi00342a010
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
LOEW, LM
中科院分区：
文献类型：
--
作者：
FLUHLER, E;BURNHAM, VG;LOEW, LM
本文研究了一系列新型电位膜探针的性能。探针都含有（氨基苯乙烯基）吡啶生色团或更高度共轭的类似物。染料的光谱特性进行了讨论的激发诱导的电荷移位从吡啶到苯胺，这种电荷移位也提供了根据电致变色机制的光谱的电压依赖性的基础。半球形双层的膜电位的光谱响应已经获得，大体上，是非常相似的所有探针测试。染料之间更细微的变化可以通过考虑结合参数、膜内深度和结构因素来部分合理化。在这个集合中最有潜力的敏感染料已被指定为二-4-ANEPPS和6-氨基-2-萘基取代母体发色团上的对苯胺基。相对荧光发射和激发响应均具有每100 mV 8%的最大值，并且这两个光谱显示出惊人的对称性。
The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; this charge shift also provides the basis for the voltage dependence of the spectra according to an electrochrome mechanism. The spectral responses to a membrane potential of a hemispherical bilayer have been obtained and, grossly, are quite similar for all probes tested. The more subtle variations from dye to dye can be partially rationalized by consideration of binding parameters, the depth within the membrane, and structural factors. The most potential sensitive dye in this collection has been designated di-4-ANEPPS and a 6-amino-2-naphthyl group in place of the p-anilino on the parent chromophore. Both the relative fluorescence emission and excitation responses have maxima of 8% per 100 mV, and these two spectra display a striking symmetry.