Increased Number of Regulatory T Cells in Children With Eosinophilic Esophagitis
Increased Number of Regulatory T Cells in Children With Eosinophilic Esophagitis
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DOI：
10.1097/mpg.0b013e3181e0817b
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发表时间：
2010-09-01
影响因子：
2.9
通讯作者：
Nadeau, Kari
中科院分区：
文献类型：
--
作者：
Fuentebella, Judy;Patel, Anup;Nadeau, Kari
目的：关于调节性T细胞（Treg）在嗜酸性粒细胞性食管炎（EoE）疾病病理学中的作用的数据有限。我们测试了EoE受试者与胃食管反流病（GERD）和健康对照组（HC）相比Treg的差异。患者和方法：我们的研究招募了经内镜检查评估的儿科患者。参与者分为3组：EoE、GERD和HC。从食管活检组织中纯化的RNA用于实时定量聚合酶链反应测定，并检测叉头盒P3（FoxP 3）mRNA表达。结果：实时荧光定量PCR检测48例食管癌患者外周血中Treg的表达，结果显示：EoE（n = 33）、GERD（n = 7）和HC（n = 8）患者外周血中Treg的表达均为CD 3 +/FoxP 3+。EoE组FoxP 3表达较GERD组和HC组高1.5倍（P < 0.05）。然后在21名受试者中研究FoxP 3在血液和组织中的蛋白水平：EoE（n = 10）、GERD（n = 6）和HC（n = 5）。各组外周血Treg及其亚群的百分比差异无统计学意义（P > 0.05）。食管组织中Treg的数量在EoE组中显著增加（平均10.7 CD 3 +/FoxP 3+细胞/高倍视野[HPF]）与其他组相比（GERD，平均1.7 CD 3 +/FoxP 3+细胞/HPF和HC，平均1.6 CD 3 +/FoxP 3+细胞/HPF）结论：EoE患者食管组织中Treg表达明显高于GERD和HC患者。本研究阐明了EoE的另一种可能机制，即免疫稳态受损。
Objectives: There are limited data on the role of regulatory T cells (Treg) in the disease pathology of eosinophilic esophagitis (EoE). We tested the differences in Treg in subjects with EoE compared with those with gastroesophageal reflux disease (GERD) and healthy controls (HC).Patients and Methods: Pediatric patients evaluated by endoscopy were recruited for our study. Participants were categorized into 3 groups: EoE, GERD, and HC. RNA purified from esophageal biopsies were used for real-time quantitative polymerase chain reaction assays and tested for forkhead box P3 (FoxP3) mRNA expression. Treg were identified as CD4+CD25hi CD127lo cells in peripheral blood and as CD3+/FoxP3+cells in esophageal tissue.Results: Forty-eight subjects were analyzed by real-time quantitative polymerase chain reaction: EoE (n = 33), GERD (n = 7), and HC (n = 8). FoxP3 expression was higher by up to 1.5-fold in the EoE group compared with the GERD and HC groups (P < 0.05). Protein levels of FoxP3 in blood and tissue were then investigated in 21 subjects: EoE (n = 10), GERD (n = 6), and HC (n = 5). The percentage of Treg and their subsets in peripheral blood were not significant between groups (P > 0.05). The amount of Treg in esophageal tissue was significantly greater in the EoE group (mean 10.7 CD3+/FoxP3+cells/high power field [HPF]) compared with the other groups (GERD, mean 1.7 CD3+/FoxP3+cells/HPF and HC, mean 1.6 CD3+/FoxP3+cells/HPF) (P < 0.05).Conclusions: We show that Treg are increased in esophageal tissue of EoE subjects compared with GERD and HC subjects. The present study illustrates another possible mechanism involved in EoE that implicates impairment of immune homeostasis.