ANTIDIABETIC POTENTIAL OF RHODIOLA SACHALINENSIS ROOT EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
ANTIDIABETIC POTENTIAL OF RHODIOLA SACHALINENSIS ROOT EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
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DOI：
10.1358/mf.2009.31.6.1380457
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发表时间：
2009-07-01
影响因子：
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通讯作者：
Duan, Yanfen
中科院分区：
文献类型：
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作者：
Gao, Dawei;Li, Qingwang;Duan, Yanfen
本研究旨在探讨高山红景天根提取物（Rhodiola sachalinensis root extract，RS）的抗糖尿病作用及其可能机制。提取物经薄层色谱分析，确定其主要成分为多糖。在链脲佐菌素诱导的糖尿病大鼠中，RS通过降低血糖显示出显著的降血糖活性（剂量为200和400 mg/kg，持续40天）。RS治疗组大鼠血清总胆固醇和甘油三酯水平低于对照组。还观察到RS治疗后糖尿病大鼠的血清胰岛素水平显著增加。此外，RS治疗降低丙二醛水平，而增加超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶活性的糖尿病大鼠的肝脏和肾脏。在此期间，RS在LD 50和单细胞凝胶电泳试验中未显示任何显著毒性。这些结果表明RS具有降血糖和降血脂活性，是一种有效的自由基清除剂，抑制脂质过氧化。RS的抗氧化和胰腺β细胞保护活性可能是观察到的RS抗糖尿病作用的主要机制。
In this study, we examined the antidiabetic effect and probable mechanisms of Rhodiola sachalinensis root extract (RS). The extract was examined by thin-layer chromatographic analysis, and the main compound was determined to be a polysaccharide. In streptozotocin-induced diabetic rats, RS showed significant hypoglycemic activity by lowering blood glucose (at doses of 200 and 400 mg/kg for 40 days). The levels of serum total cholesterol and triglycerides in RS-treated diabetic rots were lower than in control diabetic rats. A significant increase in the serum insulin levels of diabetic rats following RS treatment was also observed. Furthermore, RS treatment decreased malondialdehyde levels, while increasing superoxide dismutase, catalase and glutathione peroxidase activities of the liver and kidney of diabetic rats. At the some time, RS did not show any significant toxicity in LD50 and single-cell gel electrophoresis assays. These results indicate that RS has hypoglycemic and hypolipidemic activities and is on effective scavenger of free radicals that inhibits lipid peroxidation. The antioxidant and pancreatic beta-cell-protective activities of RS may be the main mechanisms of the observed antidiabetic effect of RS.