Changes in gene expression of Actinobacillus pleuropneumoniae in response to anaerobic stress reveal induction of central metabolism and biofilm formation
Changes in gene expression of Actinobacillus pleuropneumoniae in response to anaerobic stress reveal induction of central metabolism and biofilm formation
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DOI：
10.1007/s12275-014-3456-y
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发表时间：
2014-04
影响因子：
3
通讯作者：
Lu Li;Jiawen Zhu;Kui Yang;Zhuofei Xu;Ziduo Liu;R. Zhou
中科院分区：
文献类型：
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作者：
Lu Li;Jiawen Zhu;Kui Yang;Zhuofei Xu;Ziduo Liu;R. Zhou
胸膜肺炎放线杆菌是一种重要的猪呼吸道病原菌，在世界范围内给养猪业造成巨大的经济损失。缺氧是A.胸膜肺炎在早期感染和后期持续阶段都会发生。了解A.本研究比较了在厌氧和好氧条件下胸膜肺炎菌基因表达谱的差异。微阵列结果显示，631个基因（占总ORF的27.7%）在厌氧条件下差异表达。参与糖酵解、碳源吸收系统、丙酮酸代谢、发酵和电子呼吸转运链的蛋白质编码基因表达上调。这些变化导致细菌细胞中丙酮酸盐、乳酸盐、乙醇和乙酸盐的量增加，如代谢物检测所证实。编码参与细胞表面结构，特别是生物膜形成、肽聚糖生物合成和脂多糖生物合成的蛋白质的基因也上调。在厌氧条件下，生物膜的形成显着增强。这些结果表明，中枢代谢的诱导对于A.胸膜肺炎后转换到厌氧环境。增强的生物膜形成可能有助于这种病原体在受损的厌氧宿主组织中以及在早期定植阶段的持久性。这些发现为A.胸膜肺炎是对环境压力的反应。
Actinobacillus pleuropneumoniaeis an important porcine respiratory pathogen causing great economic losses in the pig industry worldwide. Oxygen deprivation is a stress thatA. pleuropneumoniaewill encounter during both early infection and the later, persistent stage. To understand modulation ofA. pleuropneumoniaegene expression in response to the stress caused by anaerobic conditions, gene expression profiles under anaerobic and aerobic conditions were compared in this study. The microarray results showed that 631 genes (27.7% of the total ORFs) were differentially expressed in anaerobic conditions. Many genes encoding proteins involved in glycolysis, carbon source uptake systems, pyruvate metabolism, fermentation and the electron respiration transport chain were up-regulated. These changes led to an increased amount of pyruvate, lactate, ethanol and acetate in the bacterial cells as confirmed by metabolite detection. Genes encoding proteins involved in cell surface structures, especially biofilm formation, peptidoglycan biosynthesis and lipopolysaccharide biosynthesis were up-regulated as well. Biofilm formation was significantly enhanced under anaerobic conditions. These results indicate that induction of central metabolism is important for basic survival ofA. pleuropneumoniaeafter a shift to an anaerobic environment. Enhanced biofilm formation may contribute to the persistence of this pathogen in the damaged anaerobic host tissue and also in the early colonization stage. These discoveries give new insights into adaptation mechanisms ofA. pleuropneumoniaein response to environmental stress.