Immunogenicity of liposomal model membranes sensitized with spin-labeled haptens and topographical distribution of haptens on the membranes.
Immunogenicity of liposomal model membranes sensitized with spin-labeled haptens and topographical distribution of haptens on the membranes.
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用自旋标记的半抗原致敏的脂质体模型膜的免疫原性和膜上半抗原的拓扑分布。
DOI：
10.1093/oxfordjournals.jbchem.a134111
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
T. Yasuda
中科院分区：
文献类型：
--
作者：
K. Hashimoto;K. Inoue;S. Nojima;T. Tadakuma;T. Yasuda
本文研究了以2，4-二硝基苯基-6-N-氨基己酰磷脂酰乙醇胺（DNP-Cap-PE）为半抗原的磷脂酰胆碱脂质体的体外免疫原性与半抗原在脂质膜上的分布的关系。在二硬脂酰磷脂酰胆碱脂质体中，免疫原性随着脂质体膜中胆固醇含量的增加而增加。自旋标记的DNP-Cap-PE在二硬脂酰磷脂酰胆碱脂质体中的电子自旋共振谱表明胆固醇影响自旋标记的DNP-Cap-PE在膜上的拓扑分布。在胆固醇不存在的情况下，大量的半抗原聚集在二硬脂酰磷脂酰胆碱膜上，但随着胆固醇的增加，半抗原在膜上的随机分布增加。半抗原在膜上的拓扑分布的胆固醇依赖性变化导致免疫原性的变化，即，当半抗原聚集在脂质体膜上时，免疫原性低。在膜上以适当距离排列的半抗原可能是最佳免疫应答所需的。
The relation between the in vitro immunogenicity of phosphatidylcholine liposomes containing 2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine (DNP-Cap-PE) as a hapten and the topographical distribution of the haptens on lipid membranes was studied. In distearoylphosphatidylcholine liposomes, the immunogenicity increased with increase of cholesterol content in the liposomal membranes. The electron spin resonance spectra of spin-labeled DNP-Cap-PE in distearoylphosphatidylcholine liposomes indicated that cholesterol affected the topographical distribution of spin-labeled DNP-Cap-PE on the membranes. In the absence of cholesterol, a considerable amount of haptens was clustered on the distearoylphosphatidylcholine membranes, but with increase of cholesterol, random distribution of the haptens on the membranes increased. The cholesterol-dependent change in the topographical distribution of the haptens on the membranes paralleled the change of immunogenicity, i.e., the immunogenicity was low when haptens were clustered on the liposomal membranes. Haptens arranged at a proper distance on the membranes may be required for optimum immune response.