Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria.
Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria.
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DOI：
10.1186/1556-276x-9-373
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发表时间：
2014
影响因子：
--
通讯作者：
Kim JH
中科院分区：
文献类型：
--
作者：
Gurunathan S;Han JW;Kwon DN;Kim JH
银纳米颗粒(AgNPs)因其独特的物理、化学和生物学特性，被广泛应用于抗菌、抗真菌、抗病毒、抗炎、抗血管生成等领域。本研究旨在研究银纳米粒子单独以及与常规抗生素联合应用对多种人体致病菌的抗菌和抗生物被膜作用。在这里，我们展示了一种简单、可靠、经济、绿色的合成AgNPs的方法--用芦荟叶提取物处理银离子。采用紫外-可见吸收光谱、X射线衍射仪、傅里叶变换红外光谱仪、X射线光电子能谱、动态光散射、透射电子显微镜等手段对A.Cobbe介导法合成AgNPs进行了表征。此外，通过体外杀菌试验、纸片扩散试验、生物被膜抑制试验以及对铜绿假单胞菌、福氏志贺菌、金黄色葡萄球菌和肺炎链球菌的抑制等一系列试验，评价了抗生素、AgNPs或AgNPs与抗生素的组合对铜绿假单胞菌、福氏志贺菌、金黄色葡萄球菌和肺炎链球菌的抗菌和抗生物膜活性。结果表明，与抗生素联合使用时，银纳米粒在最低浓度时有显著的抗菌和抗生物被膜作用，而在亚致死浓度下则有明显的抗菌和抗生物被膜作用。氨苄西林和万古霉素对革兰氏阴性菌和革兰氏阳性菌均有明显的增强作用。这些数据表明，将抗生素和生物银纳米粒结合起来可以用于治疗由细菌引起的传染病。这项研究提供了由A.cobe介导的AgNPs合成的抗菌和抗生物被膜作用的证据，以及它们对各种人类病原菌的增强能力。这些结果表明，AgNPs可以作为一种佐剂用于治疗传染病。
Silver nanoparticles (AgNPs) have been used as antibacterial, antifungal, antiviral, anti-inflammtory, and antiangiogenic due to its unique properties such as physical, chemical, and biological properties. The present study was aimed to investigate antibacterial and anti-biofilm activities of silver nanoparticles alone and in combination with conventional antibiotics against various human pathogenic bacteria. Here, we show that a simple, reliable, cost effective and green method for the synthesis of AgNPs by treating silver ions with leaf extract of Allophylus cobbe. The A. cobbe-mediated synthesis of AgNPs (AgNPs) was characterized by ultraviolet-visible absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), and transmission electron microscopy (TEM). Furthermore, the antibacterial and anti-biofilm activity of antibiotics or AgNPs, or combinations of AgNPs with an antibiotic was evaluated using a series of assays: such as in vitro killing assay, disc diffusion assay, biofilm inhibition, and reactive oxygen species generation in Pseudomonas aeruginosa, Shigella flexneri, Staphylococcus aureus, and Streptococcus pneumonia. The results suggest that, in combination with antibiotics, there were significant antimicrobial and anti-biofilm effects at lowest concentration of AgNPs using a novel plant extract of A. cobbe, otherwise sublethal concentrations of the antibiotics. The significant enhancing effects were observed for ampicillin and vancomycin against Gram-negative and Gram-positive bacteria, respectively. These data suggest that combining antibiotics and biogenic AgNPs can be used therapeutically for the treatment of infectious diseases caused by bacteria. This study presented evidence of antibacterial and anti-biofilm effects of A. cobbe-mediated synthesis of AgNPs and their enhanced capacity against various human pathogenic bacteria. These results suggest that AgNPs could be used as an adjuvant for the treatment of infectious diseases.